Data are presented on evaluation of use of combinations of aranosa (Ar), irinotecan (I) and cisplatin (C) in treatment of Lewis lung carcinoma. The drugs were administered i.p. simultaneously, in single doses: irinotecan 20-100 mg/kg, single dose, cisplatin 2.5 3.0 mg/kg and aranosa 100-200 mg/kg, three doses. High synergism (IP, PAr, IPAr) and sufficient tolerance were reported. Efficiency of IPAr regimen was significantly higher while hematotoxity prognosis - comparable to those of IP.

Clinical and experimental effects of neoadjuvant treatment of endometrial cancer patients with non-steroidal aromatase inhibitors: letrozole (femara, n=10, 2.5 mg/day, 14 days), anastrozole (arimidex, n=15,1 mg/day, 28 days) and exemestane (aromazine, n=13, 25 mg/day, 14 days) were compared. Administration of anastrozole was mostly frequently followed by pain relief in the lower abdomen and/or decreased rates of uterine discharge. Endometrial wall thickness (M-echo signal) decreased significantly in 60% of patients receiving anastrozole, exemestane - 58.3% and letrozole - 40%. Substantial drop in intratumoral aromatase and blood estradiol levels occurred more frequently after anastrozole and letrozole while progesterone receptor levels in tumor were markedly lower after exemestane administration. Assay of blood LH (except letrozole), FSH and cholesterol appeared to be of less relevance. On the contrary, significance of assessment of marker Ki-67 expression, which, in the case of anastrozole, dropped in 6 out of 12 patients after a 28-day course, could hardly be underestimated.

Our study involved 247 patients with histologically verified breast tumors, aged 48-89, who had received hormones - tamoxifen as first-line therapy, exemestan (second-line) for 12 months. FACT-B and FACT-G questionnaires were used to assess quality of life. Worse results were reported in tamoxifen-treated patients older than 60 years. Indices of emotional and social security in the two groups: 60-70 year-olds (10.8+/-0.96% and 14.3+/-1.27% vis-à-vis 14.8+/-1.31% and 15.6+/-1.42%, respectively) and over 70 year-olds (15.2+/-1.46% and 15.8+/-1.48%, respectively). Our evidence suggested that a large-seale complex of effective psychological rehabilitation be given, particularly, to those under 60, married andlor with minors, right from the very beginning of treatment.

Cytotoxicity and anti-EBV activity ofProteflasid (SPC Ekopharm, Kyiv) in two solvents: propylene glycol (PG) and syrup--in the culture of lymphoblastoid Raji cells were studied. It was determined that cytotoxic concentration (CC50) of Proteflasid in propylene glycol was 40 microg/ml, and in syrup--150 microg/ ml; effective concentration (EC50) of Proteflasid was equal for both solvents and was 0.1 microg/ml (under therapeutic action of Proteflasid) and 0.5 microg/ml (under prophylactic action of Proteflasid). Selectivity index (SI) ofProteflasid in PG was 400 and 80 (for therapeutic and prophylactic scheme, accordingly), 1500 and 300 (for therapeutic and prophylactic scheme, accordingly) in syrup.

The aim of the research was to study new drugs with palladium developed and synthesized at the Azerbaijan Medical University Scientific-Research Center Two compounds of palladium-4-aminopyridin ammonium tetrachlor palladium and Bi 4-aminopyridin dichlor were studied as more perspective substances which decrease growth of tumor. Water solution of 0.5% of these compounds were administrated intraperitoneally during 6 sessions. There were noticeable changes in protein metabolism, particularly in alpha(1) and alpha(2) fraction. The drug toxicity (Nedoshvina method), maximal endurance (LD(0)), medium lethal dose (LD(50)), lethal dose (LD(100)), cytogenetic activity (Rappoport method) were studied.

The main purpose of the present research is to study the efficiency of capecitabine in treatment of patients with breast cancer with liver metastasis. The investigation was carried on 44 patients with breast cancer with liver metastasis. Patients were divided into 2 groups. The first group was composed of patients (n=21) cycles of chemotherapy ( DCapLv) was carried out. The second group (n=23) was composed of patients treated upon the schema of CapLv. Patients had their diagnosis morphologically verified Metastatic involvement of the liver was diagnosed on the base of data of MRI, CT and ultrasonographic research. The obtained results were compared with the results of treatment of patients (n=54), who received the "classical" schema of FAC treatment. The investigation proved high efficiency of chemotherapy treatment schema in patients with breast cancer with liver metastasis. Proposed schema of chemotherapy seems to have had few hepatotoxic effects.

A preparation of nanocomplexes containing recombinant proteins (interferons alpha2b and beta1b, insulin, and human granulocyte colony stimulating factor) and natural polysialic acid (PSA) has been described. The incorporation of protein into the complex changes its electrophoretic mobility. Atomic force microscopy reveals the average size of 23-kD insulin complexes with PSA of 10-20 nm and demonstrates that more than 60% of glycopolymer molecules carry a single protein molecule. Experiments with cultured cells show that cytokines bound to polysialic acid retain their ability to regulate cell proliferation. Insulin bound to PSA has a prolonged hypoglycemic effect in vivo.

The aim of the research was to study the peculiarities of colonization of intestinal tract by Candida - in children with leukemia. It was found that in 32 out of 54 patients with leukaemia the quantity of intestinal KOE Candida in 1 g feces was correlated with the incidence of candidemia. The quantity of intestinal KOE/g Candida was higher in patients receiving antileukemic chemotherapy and antibacterial antibiotics than in normal subjects in patients with The quantity of Candida KOE/g feces was >10(5) cells per 1g of substrate. In control group this figure was within 10(3)-10(4). In all patients candidemia was revealed. In 8 patients who were not treated with antibiotics the quantity of Candida in feces was present to a less degree (<0.05). In the majority of cases (70%) in patients with leukaemia C.albicans was found, in 15% of cases - C. tropicalis. Along side with candidemia candidiazes of respiratory and urinary systems was revealed in children with leukaemia.

Industrial monomer acrylonitrile (AN) stimulated cancerogenesis in rats and humans. AN administration decreased an anti-tumor effect of anthracyclin antibiotic doxorubicin in rats with Pliss lymphosarcoma. A combined action of acrylonitrile and doxorubicin reduced erythrocytes and hemoglobin in rats without tumor and with Pliss lymphosarcoma more considerably than in rats with lymphosarcoma treated with doxorubicin. Hematotoxic effects of anthracyclin, doxorubicin and lymphosarcoma may be caused by stimulation of lipid peroxidation.

The study of tulosine (tamsulosine) efficacy and safety was made in 92 patients with lower urinary tract symptoms (LUTS) due to prostatic adenoma (PA). Tulosine treatment relieved both obstructive and irritative symptoms, IPSS parameters improved by 8.0 +/- 0.3 points, QoL--by 1.4 +/- 0.1 points, Qmax--by 2.6 +/- 0.3 ml/s. Ultrasound investigation found no significant changes in the adenoma size. Residual urine volume reduced from 42.4 to 19.7 ml (by 53.7%). Blood pressure lowered insignificantly. Side effects were mild. Thus, tulosine is a safe and effective drug for treatment of prostatic adenoma.

Modern immunotherapy has developed powerful tools for mounting antitumor response which nevertheless have had only limited success in clinic. Tumor cells use different mechanisms to escape from immune system. Thus, one of the reasons of unsuccessful immunotherapy might be induction of tolerance of tumor-specific cytotoxic lymphocytes by tumor cells. Previously we have demonstrated expression of HLA-E molecule by the cells of melanoma cell lines. In this paper we have studied HLA-E-dependent mechanism of melanoma cell escape from immune response.

The effects of cyclic polychemotherapy on reproductive organs were studied in female rats intraperitoneally injected (twice at 7-day intervals) with cyclophosphamide (21 mg/kg), adriamycin (2.1 mg/kg), vincristine (0.04 mg/kg), and prednisolone (2.1 mg/kg). Changes in the vaginal swabs on day 1 after polychemotherapy corresponded to estrus in 45% animals, to proestrus in 25%, and were undifferentiated in 30%. After repeated injection of the cytostatics, undifferentiated changes were found in 80% females and persisted for 6 days. Injection of the cytostatic complex led to degenerative changes and necrosis of follicular epithelial cells, edema and focal necroses of vascular endotheliocytes, which progressed after repeated polychemotherapy. By the end of 1.5 months following 2 courses of polychemotherapy, the numerical density of primary, early and late secondary follicles in the ovaries decreased and vascular sclerosis developed indicating progressive atrophy.

Antimuscarinic effects of ipratropium bromide and atropine are associated with prevention of the development of Arthus reaction, while the cytostatic effects of cyclophosphamide and doxorubicin lead to involution of the thymus and spleen, suppression of antibody production, and aggravation of inflammation, which causes edema of the ankle joint (cyclophosphamide treatment). Apoptosis of tumor cell and inhibition of inflammation can be essential for chemotherapy of malignant and autoimmune diseases.

The complexes of phospholipids nanoparticles (as the injection form of newly developed hepatoprotector phosphogliv) with the antitumor drug doxorubicin or with glucocorticoid budesonide have been investigated for their pharmacological activity in comparison with free forms of these drugs. Doxorubicin with phosphogliv revealed more antitumor and antimetastatic activity in C47B 1/6 mice with carcinoma LLC than free doxorubicin. Inhalation of budesonide with phosphogliv in vivo to quinea pigs resulted to more pronounced decrease of antigen or histamin induced bronchospasm, particularly its subacute phase, as compared with traditional powder or suspension budesonide forms. This suggests that the phosphogliv injection forms may be used not only for treatment of liver diseases, but also for delivery of a number of other drugs and consequently for optimization of their efficiency.

The application of principal components for the analysis of kinetic data obtained by optical spectroscopy is described. The use of singular value decomposition (SVD) for stable and reproducible generation of principal components, details of realization, advantages and drawbacks of the method are discussed. The described method with minor modifications may be used in a wide variety of UV-spectroscopy applications in molecular biology and biophysics. The developed method was applied to study the reaction of platinum anticancer drug, cisplatin, with DNA and methionine. Use of sensitive UV-spectroscopy allowed to study low platinum concentrations, typical for biological systems. It has been shown, that reactions of cisplatin with DNA and L-methionine generally follow the same pathway both at high and low concentrations.