To destroy Ca2+ signal system, the detached leaves of Capsicum annuum were pretreated with EGTA, a commonly used Ca2+ chelator, or La3+, an ion which competitively inhibits Ca2+ movement through plasma membrane channels. The induction of sesquiterpene cyclase gene expression in the pretreated pepper leaves under the treatments of CuCl2, HgCl2 and UV was conducted in this paper. The result showed that no hindrance of sesquiterpene cyclase enzyme activity was detected in the EGTA or LaCl3 pretreated leaves, compared to the controls treated with CuCl2, HgCl2, and UV without the pretreatment, while the pretreatment with EGTA enhanced the inductivity of the above exogenous abiotic elicitors on the enzyme activity. The sesquiterpene cyclase activity was also detected in leaves treated with EGTA only, and the result of Northern Blot analysis indicated that the enhancement of EGTA on the enzyme activity was due to the induction of EGTA on the gene transcription. It is suggested that there might exist other signal pipelines, in addition to the Ca2+ signal system in sesquiterpene cyclase gene expression in pepper leaves, and the mechanism of induction of abiotic elicitors on sesquiterpene cyclase in C. annuum differed from that of biotic elicitors.

To evaluate the activity in vivo of 3 phosphorothioate antisense oligonucleotides, HCV363, HCV279 and HCV349 using nude mice xenograft models based on the establishment of HCV 5' NCR transgenic cellular model (HepG2.9706).

To explore the effect of HCV core protein on vascular endothelial growth factor (VEGF) in HepG(2) cells.

Nine nitroaromatic hydrocarbons (NAHs) induction of EROD activity was investigated by an in vitro assay based on carp (Cyprinus carpio) hepatic microsome system. All nine NAHs tested were found to induce the activity of EROD. There was dose-effect relationship between EROD activity and concentrations of NAHs. It was found that EROD activity was different, when different groups substituted at the same position of benzene ring, or the same group was at different position of benzene ring.

To study the specific inhibition of HBV gene expression by antisense oligonucleotide (ASON) targeted by galactosylated poly (L-lysine) (Gal-PLL).

To investigate whether ethanol and isopentanol could modulate the drug metabolism by regulating uridine diphosphate glucuronosyltransferase (UGT) expression in rat livers and cultured rat hepatocytes.

To use the retrovirus vector Tet-On regulation system to control the expression of TPO and luciferase gene by doxycycline (Dox).

To observe the expression of CD14 mRNA and its protein in rat hepatocytes during endotoxemia.

To identify the receptor of hyaluronic acid mediated motility (RHAMM), expression pattern(s) in the gastric mucosa with various lesions and to elucidate the effect of hyaluronic acid(HA) on in vitro RHAMM expression.

To elucidate the cause of abnormal Rb expression in esophageal cancer (EC).

To study oocyte maturation of human fetal preantral follicles in vitro, the effects of different factors on oocyte maturation.

To investigate the effect of laminin antisense RNA on laminin antisense RNA on the expression of E-cadherin, alpha-, beta-, gamma-catenins and focal adhesion kinase(FAK) stimulated by transforming growth factor beta(1)(TGFbeta(1)).

To explore the role of airway smooth muscle cells (ASMC) stimulated by inflammatory factors in the development of asthma and bronchial hyperresponsiveness (BHR) and the possible mechanism of low dose theophylline in the treatment of airway inflammation, the effects of GM-CSF on endothelin-1 and endothelin converting enzyme (ECE) gene expression in human ASMC and theophylline on their expressions were investigated.

To investigate whether nonenzymatic glycated end products (AGEs) have effects on the expression and bioactivity of plasminogen activator inhibitor-1 (PAI-1), one of the seripin proteinases, which lead to extracellular matrix (ECM) degradation in cultured human mesangial cells.

Angiotensinogen is the only known precursor of vasoactive angiotensin II and also one of the acute phase proteins. This study was intended to understand the regulation of angiotensinogen gene expression induced by IL-6.

To investigate the antitumor mechanism of interleukin-2 (IL-2) and interleukin-6 (IL-6) gene therapy.

To investigate the apoptosis of HL-60 cells induced by 9-cis retinoic acid (9-cis RA), and illustrate the possible molecular mechanism.

To define the prevalence of acquired red cell enzymopathy in leukemia and MDS patients and explore its clinical significance.

To evaluate whether the growth inhibition by retinoic acid and RAR alpha mRNA expression levels were affected by the change of ER expression.

To study the effect of IFN-alpha on the expressions of perforin and granzymes in IL-2-activated-lymphocytes.