To study the effect of triptolide on airway smooth muscle (ASM) proliferation and expressions of c-fos and c-jun in asthmatic rats.

To observe the effect of nitric oxide on expression of matrix metalloproteinase and tissue inhibitor of metalloproteinase in asthmatic rats.

To study the effect of losartan on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism.

To engineer 293 cells to an artificial beta cell line which secrets insulin in response to stimulation by doxycycline.

To investigate the regulation of human progesterone receptor isoforms A and B in uterine endometrial carcinoma by estrogen and insulin-like growth factor-1 (IGF-I) so as to provide theoretical basis for clinical hormone treatment of uterine endothelium cancer.

To isolate and clone the differentially expressed genes in vascular endothelial cell induced by high density lipoprotein (HDL) so as to study the anti- atherogenesis molecular mechanism of HDL.

To observe the in vitro effect of compound 861 (Cpd 861) on tissue inhibitor of metalloprotenase 1 (TIMP1) mRNA levels of HSC-T6 cell.

To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.

To investigate PML gene and protein expression and localization in leukemia cell lines.

To study the reversal effect of nomegestrol acetate (NOM) on mutidrug resistance (MDR) in MCF7/ADR and its mechanism.

Even though phenylacetate (PA) bas been shown to inhibit the growth and induce differentiation in rat C6 glioma cell line, its mechanisms are still poorly understood. This study is aimed to identify which Hox gene is related to glioma and to observe the change in expression on mRNA level as treated by phenylasetate.

NIH3T3 cells transfected with inducible expression vectors harboring the protein tyrosine phosphatase alpha (PTPalpha) gene were used as a model to study the mechanism of tumor formation, by which the changes of phenotypes in inducible cells were studied. When NIH3T3 cells transfected with PTPalpha were induced for 24 hours, the expression levels of PTPalpha in inducible cells was higher than those in uninducible cells as judged by RT-PCR and Western blotting; the expression level of Src in inducible cells was the same as those in uninducible cells, but activity of Src kinase in inducible cells was higher than those in uninducible cells.The level of phosphated tyrosine in Src was reduced in inducible cells. The malignant changes of phenotypes in the inducible cells were verified by flow cytometry and electron microscope. The results show that 24 h induction of PTPalpha could initiate the transformation of NIH3T3 cells transfected with PTPalpha inducible expression vectors, and the transformation may be associated with activated Src where pTyr(527) in the C tail was dephosphorylated by increased expression level of PTPalpha in the cells.

To study the effect of gene amplification and selection system with DHFR plus GS and DHFR or GS gene on the foreign gene expression.

To investigate the effect of ursodeoxycholic acid (UDCA) on liver regeneration after 70% partial hepatectomy (PH) in bile duct obstructive (BDO) rats.

To observe the expression of CD(14) protein and CD(14) gene in liver sinusoidal endothelial cells (LSECs) of rats during endotoxemia and the role of CD(14) protein in the activation of lipopolysaccharide (LPS)-induced LSECs.

RNAs of the leaves in Avicennia marina, which cultured in 50@1000 and 0@1000 salinity condition respectively, were isolated for mRNA differential display analysis. Screened by OligodT12 GC and eight 10-oligonucleotide arbitrary primers, differential cDNA fragments-csrg1(600 bp), csrg2(550 bp), csrg3(480 bp), only appeared in the leaves of Avicennia marina cultured in 50@1000 salinity condition. After detected by RNA dot hybridization, only csrg1 appeared the difference between the RNAs of the leaves in Avicennia marina cultured in 50@1000 and 0@1000 salinity condition respectively, and csrg1 was confirmed as the salt-tolerant cDNA. csrg1 was cloned and sequenced. After searching in Genbank, there were no similar sequences reported.

To clarify the possible effect of reactive oxygen species such as hydrogen peroxide on progression of human colorectal cancer.

We study the regulation of human progesterone receptor isoforms A and B in uterine endometrial carcinoma cell line by different concentration of human insulin-like growth factor-I (IGF-I) for different time, to investigate the roles of IGF-I and progesterone receptor isoforms in uterine endometrial carcinoma.

To study the effect of tumor necrosis factor-alpha (TNF-alpha) on type I inositol 1, 4, 5-triphosphate receptor (IP(3) R) expression in rat glomerular afferent arterioles smooth muscle cells (RASMC).

The present study further revealed the teratogenic mechanism of methylmercury chloride (MMC) during rat neurulation by means of nonradioactive in situ hybridization (ISH), semiquantitative analysis of immunohistochemical staining and in vivo teratogenic test. The main aim was to teat the hypothesis that iNOS, HSP70, NT, TGF-beta and Bcl-2 genes contribute to MMC-induced day 9.5 embryonic damages. Results showed there were no obvious poisoning signs and death of pregnant female rats injected intraperitoncally with 0, 0.2, 0.4, 0.8, 1.6 and 3.2 mg/kg MMC. While the doses increased, the total morphological scores decreased gradually, and the rates of embryo deformity and development delay increased step by step to 34% and 76% respectively. The levels of iNOS mRNA and protein and HSP70 mRNA increased, and NT mRNA with its protein became down, all these changes were concentration dependent. In addition, MMC could inhibit the level of TGF-beta mRNA, but no obvious influence on the levels of Bcl-2 mRNA and Bcl-2 protein. On the basis of parallel findings from embryos, genes and proteins, abnormal expression of genes in transcriptional level might be related to MMC-induced teratogenic insult.