To investigate differential gene expression in apoptotic cells induced by As(2)O(3), and identify novel apoptosis-related genes.

To investigate the effect of recombinant human hepatopoietin (rhHPO) and partial hepatectomy on rapidly induced expression of immediate early gene.

Peptide nucleic acid(PNA) is a kind of artificial DNA mimic. PNA hybridizes with DNA or RNA by means of Watson-Crick's base-pairs complementary with high stability, affinity and selectivity. PNA not only regulates. DNA replication, but also adjusts DNA transcription (or reverse transcription) and translation. Many applications have been explored as a new kind of molecular biological tool and a gene-targeting strategy.

An analysis of the induction of sesquitèrpene cyclase gene expression and antioxidant enzymes in Capsicum annuum by exongenous salicyclic acid (SA) pointed out that the sesquiterpene cyclase gene expression in detached pepper leaves was induced by 0.5-4 mmol.L-1 of exongenous SA, whereas the enzyme activity and accumulation of cyclase mRNA were much lower, and it took a longer time (36 h) for the gene to be induced to express after SA treatment, comparing to other elicitors such as UV and fungi treatment. The activities of SOD and POD were enhanced, while the CAT activity was inhibited to some degree. Consequently, H2O2 content was increased in SA treated pepper leaves. H2O2 accumulation was related to the integrated influences from antioxidant enzymes.

To study the effect of combination of eicosapentaenoic acid (EPA) and retinoic acid (RA) on the proliferation and differentiation of HL-60 cells and its mechanisms.

To study the role of transforming growth factor-beta(1) (TGF-beta(1)) in the pathogenesis of chronic bronchitis and emphysema.

To study the effect of triptolide on airway smooth muscle (ASM) proliferation and expressions of c-fos and c-jun in asthmatic rats.

To observe the effect of nitric oxide on expression of matrix metalloproteinase and tissue inhibitor of metalloproteinase in asthmatic rats.

To study the effect of losartan on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism.

To engineer 293 cells to an artificial beta cell line which secrets insulin in response to stimulation by doxycycline.

To investigate the regulation of human progesterone receptor isoforms A and B in uterine endometrial carcinoma by estrogen and insulin-like growth factor-1 (IGF-I) so as to provide theoretical basis for clinical hormone treatment of uterine endothelium cancer.

To isolate and clone the differentially expressed genes in vascular endothelial cell induced by high density lipoprotein (HDL) so as to study the anti- atherogenesis molecular mechanism of HDL.

To observe the in vitro effect of compound 861 (Cpd 861) on tissue inhibitor of metalloprotenase 1 (TIMP1) mRNA levels of HSC-T6 cell.

To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.

To investigate PML gene and protein expression and localization in leukemia cell lines.

To study the reversal effect of nomegestrol acetate (NOM) on mutidrug resistance (MDR) in MCF7/ADR and its mechanism.

Even though phenylacetate (PA) bas been shown to inhibit the growth and induce differentiation in rat C6 glioma cell line, its mechanisms are still poorly understood. This study is aimed to identify which Hox gene is related to glioma and to observe the change in expression on mRNA level as treated by phenylasetate.

NIH3T3 cells transfected with inducible expression vectors harboring the protein tyrosine phosphatase alpha (PTPalpha) gene were used as a model to study the mechanism of tumor formation, by which the changes of phenotypes in inducible cells were studied. When NIH3T3 cells transfected with PTPalpha were induced for 24 hours, the expression levels of PTPalpha in inducible cells was higher than those in uninducible cells as judged by RT-PCR and Western blotting; the expression level of Src in inducible cells was the same as those in uninducible cells, but activity of Src kinase in inducible cells was higher than those in uninducible cells.The level of phosphated tyrosine in Src was reduced in inducible cells. The malignant changes of phenotypes in the inducible cells were verified by flow cytometry and electron microscope. The results show that 24 h induction of PTPalpha could initiate the transformation of NIH3T3 cells transfected with PTPalpha inducible expression vectors, and the transformation may be associated with activated Src where pTyr(527) in the C tail was dephosphorylated by increased expression level of PTPalpha in the cells.

To study the effect of gene amplification and selection system with DHFR plus GS and DHFR or GS gene on the foreign gene expression.

To investigate the effect of ursodeoxycholic acid (UDCA) on liver regeneration after 70% partial hepatectomy (PH) in bile duct obstructive (BDO) rats.