To explore the effect of N-acetyl L-cysteine (NAC) on expressions of matrix metalloproteinases-2, 9 (MMP-2, MMP-9) in lung fibroblasts of SiO(2) exposed rats.

To investigate the effects of irbesartan for heart protection and on heart nitric oxide (NO) system in diabetic rats.

To study the mechanisms of Shengqing Capsules, a compound Chinese herbal medicine for dispersing stagnated liver qi and promoting bile flow, in prevention and treatment of pigment gallstone.

To explore the mechanisms of the effects of Runing Recipe in anti-invasion and anti-recurrence of breast cancer by experimental research in vitro.

The accumulation of unfolded proteins in the endoplasmic reticulum (ER) lumen causes ER stress response in eukaryote cells, activates the unfolded protein response (UPR) and consequently induces the expression of ER-located molecular chaperones (such as BiP, calnexin, etc.). In this study, the full-length small heat-shock protein of endoplasmic reticulum cDNA (ER-sHSP) under the control of CaMV 35S promoter was introduced into tomato by Agrobacterium tumefactions mediated method (Fig. 1, Table 1). Overexpression of ER-sHSP enhanced the resistance of the transgenic plant to tunicamycin (Fig. 2, Table 2), an inducer of ER stress. Tunicamycin treatment induced an increase in mRNA level of BiP and calnexin in plant cell, but mRNAs of these two genes in transgenic plants were accumulated to a much smaller extent than those in non-transgenic plants (Fig. 3). These results indicated that ER-sHSP could alleviate the ER stress and may be involved in the UPR signal transmission pathway.

To evaluate the effect of a novel anti-human DR5 monoclonal antibody (mAb mDRA-6) on the apoptosis of human hepatocyte HL7702 cell lines.

To investigate the influence of different stimulators on cytokines secretion of tumor-draining lymph node (TDLN) cells and study antitumor effects and mechanism of TDLN cells.

To study the effect of quercetin on the growth and apoptosis of human gastric carcinoma cell line MGC-803.

To investigate the effect of IFN-alpha on expression of MHC class I chain-related protein A (MICA) in the cervical carcinoma cell lines.

The study is to investigate the effect of IL-10, IFN-gamma on viability and IL-10 receptor expression of human decidual stromal cells (DSC) in early pregnancy. The vitality of DSC was detected by MTT. IL-10R1 and IL-10R gene expression of DSC were detected after treated by different concentrations of IL-10, IFN-gamma at 15min, 30min, 45min, 60min. The vitality of DSC was significantly enhanced by the higher dose of IL-10 (100-10ng/ml) (P<0.05), and there was no significant stimulative role induced by the lower dose of it(0.10-1ng/ml)(P>0.05). The viability of DSC was inhibited by the highest dose of IFN-gamma (1000ng/ml) (P<0.01), on the contrary, its viability was stimulated by the lower dose of IFN-gamma (10ng/ml) (P<0.05). IL-10R1 was highly expressed by IL-10 of 10ng/ml within 15min, and significantly reduced at 30min, then disappeared within 45min; while IL-10R1 expression was not induced by IL-10(1ng/ml) of lower concentration throughout 60min; IL-10R1 was briefly and lowly expressed by IFN-gamma of 100ng/ml, which effect on DSC at 45min; The expression of IL-10R1 was moderate level induced by IFN-gamma of 10ng/ml within 30min,and reduced at 45min,then disappeared at 60min. There was no significant difference of IL-10R2 expression (P>0.05) between treatment and not with the above-mentioned cytokines. IL-10, IFN-gamma may play an important role of immune regulation in early pregriancy by influencing IL-10R1 expression and vitality of DSC.

Specific primer sequences were designed on the basis of converse motifs of cloned resistance genes and subsequently used as PCR primers to amplify the resistance gene analog (RGA) in wheat (Triticum aestivum L.) Xinong 88 which possesses rust disease resistance. The amplified products of WRGA1, WRGA2 and WRGA14 were cloned and sequenced. The wheat RGAs contain some conserved motifs such as Kinase-2a, Kinase-3a and HD presenting in known NBS-LRR resistance genes from other plants and share between 46.0%-9.9% amino acid sequence identity with them. The amino acid sequence identity among three RGAs is 80.7%-56.8%. The WRGA1 expression was induced and detected by Northern blotting, which is positively regulated by salicylic acid.

To explore modulation of the molecular adjuvant C3d in the hCGbeta -C3d3 fusion protein in costimulatory molecule expression on Raji cells by linking to the surface molecule CD21. Raji cells, B cell line, were incubated with the purified hCGbeta-C3d3, hCGbeta or C3d3 protein for 15 hours respectively in the interference of anti-CD21 monoclonal antibody or not. The culture concentration of each group was 10, 30, or 90 microg/ml respectively. The expression of both B7-1 and B7-2 on Raji cells were analyzed by flow cytometric assay. It was observed that compared to hCGbeta or C3d3 protein, hCGbeta-C3d3 up-regulated both B7-1 and B7-2 expression on the Raji cells, and the effect was in a dose-dependent manner. The up-regulation effect was blocked efficiently by anti-CD21 mAb. The results showed that molecular adjuvant C3d may up-regulate both B7-1 and B7-2 expression on Raji cell via C3d/CD21/CD19 complex, thus enhance the antigen presentation of B cell, and the interaction between B cell and T cell.

To investigate the mechanism of tazarotene against active psoriasis vulgaris.

To identify proteins associated with growth inhibitory effects of progesterone in Ishikawa endometrial adenocarcinoma cell line (Ishikawa).

To study the effects of Flutamide (Flu) on the full gene expression of early testis development of embryonic period in male SD rats.

To investigate the inhibitory effects of RNAi expression vector suppressing the expression of survivin and inducing the apoptosis of pancreatic cancer cell PANC-1.

To study the genes associated with CQ (Chloroquine) increased radiosensitivity in radioresistant MDA-MB 231 cells.

To investigate the inhibition effect of leukemic bone marrow stromal cells (BMSCs) on daunorubicin (DNR) induced apoptosis of human Jurkat cell line, and analyze the differentially expressed genes between Jurkat cells cocultured with leukemic BMSCs or without.

To investigate the effects of interferon (IFN) on the natural killer (NK) cytotoxicity and the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and other apoptosis-inducing genes in peripheral blood mononuclear cells (PBMNC) from polycythemia rubra vera (PV).

To elucidate effects of histone deacetylase inhibitors on cell cycle of leukemia cell lines and investigate its molecular mechanisms.