To determine the effect of compound Rhizoma Smilacinus granules (CRSG) on the expression of Bcl-2 and Bax gene in A549 cell lines, and to explore the mechanism of CRSG on non-small cell lung cancers.

To study the effect of methotrexate (MTX), a folic acid antagonist which can lead to folic acid deficient, on the cardiac development and on the expressions of BMP2b and HAS2 in zebrafish.

To evaluate the effect of rosiglitazone (ROS) on integrin beta1 expression and apoptosis of proximal tubular cell exposed to high glucose.

To explore the role of IL-8 in the pathogenesis of chronic obstructive pulmonary disease (COPD) and the effect of glucocorticoid on COPD.

To observe the effect of platelet activating factor (PAF) antagonist, ginkgolide B (GB), on the expression of glial fibrillary acidic protein (GFAP) in astrocytes during focal cerebral ischemia, of which the mechanism will be explored too.

To test the effect of N-acetylcysteine (NAC) on the mice with infection associated preterm labor.

To identify the expression of Cyclooxygenase-2 (Cox-2) in lung tissues and its potential role in pulmonary microcirculation disorder in rats with acute pancreatitis.

To demonstrate the molecular mechanism of poor prognosis in HER-2 overexpressing breast carcinoma, to study the correlation between angiogenesis/lymphangiogenesis and HER-2, and the effect of them in the progression and prognosis, so as to provide novel molecular markers for lymph node metastasis and prognosis in breast carcinoma.

To study the growth regulation pathway and the mechanism of acquired resistance to tamoxifen (TAM) in breast cancer cells.

To study selective killing effect of herpes simplex virus-thymidine kinase/ganciclovir (Hsv-tk/GCV) driven by human telomerase catalytic subunit (hTERT) promoter on lung cancer cell line A549 in vitro.

To study the changes of the center of cellular immune thymus and the interference of Jinhong Tablet, a compound traditional Chinese herbal medicine, during the course of cholangitis.

cDNA microarray technique was used to study the mechanisms of liver injury caused by Huangyaozi at level of gene expression based on the biological functions of those differentially expressed genes. For the mice taking Huangyaozi for 15 days,there were 83 differentially expressed genes,among them 18 genes were up-regulated and 65 genes were downregulated, and the increase of serum ALT,AST and tissue total protein were notable. For the mice taking Huangyaozi for 30 days, there were 1658 differentially expressed genes,among them 917 genes were up-regulated and 741 genes were down-regulated. And the liver index, serum ALT and tissue total protein were all increased significantly but AST,AKP decreased significantly. There were 45 common differentially expressed genes in the two groups,among them 9 genes were upregulated,while the other 36 genes were down-regulated. According to the differentially expressed genes with different duration for taking Huangyaozi, the phased change about occurrence and development of liver injury was analyzed. The results indicated that the gene expression profile of mouse liver cell could be changed significantly by taking Huangyaozi, which might be important for elucidating the mechanisms of liver injury caused by Huangyaozi.

To observe the pathological characteristics of the rejection of rat cardiac allograft, study the effects and mechanisms of sinomenine on the rejection of rat cardiac allograft.

To investigate the effect and mechanism of Qingdu Suppository (QDS) in suppressing the growth of SiHa cell of cervical carcinoma on the cytobiologic and molecular biologic level.

To interfere in the Tca8113-CDDP cell line with siRNA of cyclin D1 and to investigate time and dose dependent gene silencing effect of siRNA of cyclin D1.

Semipermeable membrane devices (SPMDs) in combination with in vitro ethoxyresorufin-O-deethylase (EROD) assay was used to assess the aryl hydrocarbon receptor (AhR) effects in aquatic system. In present work, polycyclic aromatic hydrocarbons (PAHs) were selected as target compounds and SPMDs were deployed simultaneously with caged crucian carp for 32 days at five sites in Meiliang Bay, Taihu Lake. The concentrations of PAHs in the SPMD dialysates and fish tissues were analyzed by gas chromatography-mass spectrometry. In addition, the EROD activity of SPMD dialysates was assayed with H4IIE rat hepatoma cells and the induction of EROD activity in pancreas and liver of fish was also assayed. It was found that dialysates from SPMDs with longer exposures generally induced stronger activity and the toxic potency of dialysates expressed as bioassay-derived 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents ranged from 3.8 pg/g to 6.2 pg/g for 32 days exposure. A correlation of toxicity equivalency quantity (TEQ) with chemical analysis and in vitro bioassay-derived TEQ(bio) of SPMD dialysates yielded an R2 of 0.88, therefore, indicated that PAHs were one of the important AhR agonists in the water. Empirical evidence suggests that PAHs can account for about 40% to 50% of the potency observed. Moreover, a good correlation was observed between the results of EROD bioassay in vivo and EROD bioassay in vitro (R2 = 0.62), thus reflected that these two methods can be complementarily each other. It is, therefore, suggested that the SPMD technique combined with chemical analyses and in vitro EROD assay might be a valuable monitoring tool to assess the levels and effects of AhR agonists in water ecosystem.

To investigate the effects of sex hormones on sarcoplasmic reticulum (SR) Ca(2+)-ATPase activity and gene expression in the genioglossal muscle of the aged rats, and to explore the possible mechanism related to the upper airway stability.

To investigate the effects of dexamethasone (DEX) on the expression of intercellular adhesion molecule-1 (ICAM-1) in orbital fibroblasts (OF) from patients with thyroid associated ophthalmopathy (TAO).

To evaluate the effects of recombined human Neuregulin on the contractibility of cardiac muscles of Rhesus Monkeys with pacing-induced heart failure and to reveal the possible mechanisms involved.

To investigate the sensitivity to Adriamycin of down-regulated cells expressed hOGG1 gene which target to oxidative DNA damage and repair, and provide more experimental evidence of sensitizing the response of tumor to chemotherapy.