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7121. [In vitro proliferation and differentiation of bone marrow stem cells of aplastic anemia patients].
To investigate the quality of bone marrow stem cells from aplastic anemia patients and their in vitro responses to hemopoietic growth factors(HGF).
7122. [Inhibition of hematopoiesis by sera from patients with myelodysplastic syndromes].
To explore the inhibitory effect of sera from patients with myelodysplastic syndromes (MDS) on the growth of normal hematopoietic progenitor cells.
7123. [Studies on the expression of complement regulatory proteins in three kinds of stem cell defect anemias and its clinical implications].
To investigate the expression of membrane associated complement regulatory proteins (CRP)--CD55 and CD59 on the peripheral blood cells in patients with paroxysmal nocturnal hemoglobinuria (PNH), aplastic anemia(AA) and myelodysplastic syndrome (MDS) and its clinical implications.
7124. [Advances in the study of hematopoietic cell apoptosis in multiple myeloma].7125. [Are CD34- cells the more primary hematopoietic stem cells?].7126. [The cytologic ultrastructure and cytochemical features of human dendritic cells from peripheral blood].
To observe the cytomorphological, cellular ultrastructure and cytochemical features of the dendritic cell (DC) enriched populations in vitro.
7127. [Studies on hematopoietic reconstitution by ex vivo expanded human cord blood hematopoietic stem/progenitor cells in SCID mice].
To elucidate the effect of the combination of cytokines on ex vivo expansion and self-renewal potential of human umbilical cord blood hematopoietic stem/progenitor cells.
7128. [Analysis of immunophenotype of hematopoietic stem/progenitor cells in human umbilical cord blood with flow cytometry and its significance].
To study the difference of immunophenotype of hematopoietic stem/progenitor cells (HSPCs) between human umbilical cord blood(UCB) and bone marrow(BM).
7129. [Analysis of short tandem repeats with fluorescence labeling polymerase chain reaction to document engraftment following allogeneic bone marrow transplantation].
In order to determine the status of engraftment following allogeneic bone marrow transplantation (allo-BMT) and predict the outcome, short tandem repeats (STR) were analysed by fluorescence labeling polymerase chain reaction (PCR).
7130. [Membranous nephropathy after allogeneic peripheral blood hematopoietic stem cell transplantation--clinicopathologic manifestations of a rare chronic GVHD].
To investigate the clinicopathologic characteristics of renal chronic graft versus host disease (GVHD) after allogeneic peripheral blood hematopoietic transplantation(HSCT).
7131. [Establishment of cord blood stem cell bank and its clinical application].
To establish unrelated cord blood stem cell bank for providing potential grafts for stem cell transplantation in the treatment of some malignant and nonmalignant disorders.
7132. [A preliminary result of treatment of progressive multiple sclerosis with autologous peripheral blood stem cell transplantation in China].
To explore the clinical effect for treatment of progressive multiple sclerosis (PMS) by autologous peripheral blood stem cell transplantation (Auto-PBSCT).
7133. [Cytomegalovirus enteritis in recipients of allogeneic peripheral blood stem cell transplantation].
To report 6 cases of cytomeganlovirus (CMV) enteritis in allogeneic bone marrow transplantation (allo-BMT) or peripheral blood stem cell transplantation(PBSCT) recipients and the outcome after treatment.
7134. [Advances in the study of the effects of mesenchymal stem cells on osteogenesis].7135. [Regulation of gene co-expression after bicistronic retrovirus-mediated gene transfer into human cord blood CD34+ cells].
To compare internal SV40 early promoter with IRES of the in regulatory effect on gene co-expression after retrovirus-mediated gene transfer into human cord blood CD34+ cells.
7136. [Establishment of banks of hematopoietic stem cells from umbilical blood].7137. [Engineering of hematopoietic cells and the hematopoietic reconstitution of expanded cells in SCID mice].
To elucidate the roles of cytokines for ex vivo expansion and orderly differentiation of hematopoietic progenitor cells, and the capacity of hematopoietic reconstitution of the expanded cells.
7138. [ENU induced mutagenesis-large-scale study of gene mutagenesis and function].
ENU mutagenesis is becoming one of the most powerful approaches for large-scale study of gene function. The mechanism of ENU mutagenesis, factors that affect mutation rates, the strategies of the ENU mutagenesis, screening for the phenotypes, related researches and recent progresses were introduced.
7139. [Experimental observation of antagonism of polyaspartic acid against gentamicin-induced phospholipidosis in cochlea of guinea pig].
To observe the effect of polyaspartic acid (PAA) on gentamicin-induced disturbance of phospholipid metabolism in cochlea of Guinea pig.
7140. [The medium conditioned incubation with rat heart cells maintains the properties of ES cells].
17 kinds of conditioned media were selected by plating tests, propagation and other tests with two cell lines, C-19-2 and MESPU-13. The results suggested that the rat heart cells conditioned media (RH-CM) can inhibit the spontaneous differentiation effectively, maintain the normal diploid karyotypes and promote adherence and proliferation of mouse ES cells. The ES cells were propagated in RH-CM to the 20th passage remaining their pluripotent in vivo and in vitro differentiation ability. RH-CM can be used as supplement of ES cell media. ES cells which are cultured in media containing 70% RH-CM and on PMEF feeder can maintain their undifferentiation state and diploid karyotype. RT-PCR detection suggested that there was mLIF expression in rat heart cells.
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