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6741. [Recent advances in islet transplantation and pancreatic stem cell research].
This paper reviewed recent advances in pancreatic islet transplantation research, including islet isolation, purification, culture, cryopreservation and immunoisolation. Latest progresses in induction of pancreatic stem cell and embryonic stem cell to differentiate into insulin-producing islets were also introduced. On the basis of the present situation and future development of islet transplantation-based therapies for diabetes, the author thought that allogeous islet transplantation is a main choice for type I diabetes today and pancreatic stem cell transplantation for tomorrow.
6742. [Experimental research on spine fusion induced by tissue engineered bone].
作者: Guang-heng Li.;Xiao-kui Hou.;Xiang-fu Wu.
来源: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003年25卷1期39-42页
To construct a reasonable substitute for the autograft bone in vitro and transplant it back into the rabbit models to induce the spine fusion.
6743. [An experimental study of demineralized bone matrix to repair bone defects as a scaffold of tissue engineering].
作者: Ming-xue Sun.;Shi-bi Lu.;Ji-fang Wang.;Jing-xiang Huang.;Zheng Yu.;Bin Zhao.
来源: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003年25卷1期32-5页
To evaluate application of the sponge of demineralized bone matrix (SDBM) in tissue engineering of bone.
6744. [The regulatory effect of human bone morphogenetic protein 7 gene transfer on the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells].
作者: Dan Jin.;Guo-xian Pei.;Ke Wang.;Kuan-hai Wei.;Bin Chen.;Yu Qin.
来源: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003年25卷1期22-5页
To detect the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells (BMSc) transferred by retroviral vector carrying human bone morphogenetic protein 7 (hBMP-7) gene.
6745. [Study on the role of superoxide anion in promoting proliferation and transformation of rat liver oval cell line WB-E344].
To study the role of superoxide anion (O2.-) in promoting proliferation and transformation of rat liver oval cell strain WB-F344.
6746. [Effect of zinc phthalocyanine-mediated photodynamic therapy on bone marrow purging, an experimental study].
To probe into the purging effects of zinc phthalocyanine-mediated photodynamic therapy (PDT) on simulated remission bone marrow grafts of chronic granulocytic leukemia.
6747. [Overexpression of STAT3 in COS7 cells causes prominent morphological changes].
作者: Ye Yan.;Cai-Hong Xia.;Xin-Min Cao.;Nai-He Jing.
来源: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003年35卷8期717-22页
Signal transducer and activator of transcription 3 (STAT3) plays a central role in mediating signals related to cell growth, differentiation, survival and movement. In this report, the results of immunocytochemistry and Western blot analysis showed that the active form of Stat3 protein existed in COS7 cells. The endogenous Stat3 protein could also induce the expression of m67-sequence-directed reporter genes. The transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. The overexpression of Stat3 protein in COS7 cells caused prominent cell morphological changes. These cells had a much larger cell body, extended long processes with branches, lamellipodia and filopodia. These results suggest that Stat3 protein may play important roles in cell adhesion, migration and cytoskeleton reorganization.
6748. [Induced differentiation of neural stem cells from subependymal zone into retinal cells in vitro].
作者: Hui-ping Yuan.;Jian Ge.;Yong-heng Duan.;Yan-mei Wang.;Li-na Wang.;Bin-bin Yang.
来源: Zhonghua Yan Ke Za Zhi. 2003年39卷6期357-60页
To study the cultivation and differentiation of neural stem cells (NSC) in vitro and observe the effects of retinal cell-conditioned medium on the differentiation of NSC.
6749. [Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells].
作者: Shu-xun Hou.;Da-ming Sun.;Gui-xin Du.;Yi-gang Tong.;Xiao-bing Fu.
来源: Zhonghua Wai Ke Za Zhi. 2003年41卷6期445-8页
To construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.
6750. [Changes in peripheral lymphocyte subfraction in cytomegalovirus infections during the early stage of allogenic peripheral blood stem cell transplantation].
作者: Tai-Sheng Li.;Hai-Yan Qin.;Hu Chen.;Min Jiang.;Huan-Ling Wang.;Ai-Xia Wang.
来源: Zhonghua Nei Ke Za Zhi. 2003年42卷6期396-9页
To study the alterations and the clinical significance of peripheral lymphocyte subsets in patients with active cytomegalovirus (CMV) infection during the early stage of allogeneic peripheral blood stem cell transplantation (PBSCT) and to investigate the influence of active CMV infection on immunity.
6751. [Detection of hepatic progenitor cells in patients with severe hepatitis and their distribution].
作者: Zhong-jie Hu.;Zhen-wei Lang.;Chen-zhao Song.;Shi-jie Zhang.
来源: Zhonghua Gan Zang Bing Za Zhi. 2003年11卷7期394-7页
To identify hepatic progenitor cells (HPCs) in patients with severe hepatitis (SH) by detecting their markers and investigate the features of their distribution and location.
6752. [The application of endothelial progenitor cells (EPC) in the cellular and gene therapy of cardiovascular-disease].6753. [Research progress in the study of neural stem cell derived from spinal cord].6754. [Piwi gene family: the important gene modulating the divisions of stem cell].6755. [Progress in the study of muscle satellite cells].6756. [The mechanisms of a new plant lectin FRIL from dolichos lablab that preserves quiescent hematopoietic stem cells in suspension culture].
Lectins, proteins that bind sugar moieties of glycolipids and glycoproteins, occur in all organisms and are especially abundant in many plants. Plant lectins probably have multiple roles related to their ability to act as recognition molecules. Here we present a review on the biological characterization of a new plant lectin in red kidney bean named Flt3 receptor-interacting lectin(FRIL), and focus on the mechanisms that FRIL can preserve quiescent hematopoietic stem cells in suspension culture.
6757. [Clinical application for reference strand mediated conformation analysis system in HLA-A genotyping].
作者: Dan-dan Zhao.;Yu-ying Sun.;Yong-zhi Xi.;Li Jin.;Nan Liu.;Fei Liang.
来源: Zhonghua Yi Xue Za Zhi. 2003年83卷8期654-7页
To establish and stabilize a new HLA typing strategy, reference strand mediated conformation analysis (RSCA), and to conform its advantages by using HLA-A as target gene.
6758. [Effects of collagen I coating on the porous poly-lactide-co-glycolid on adhesion, proliferation, and differentiation of mesenchymal stem cells].
作者: Gang Liu.;Yun-yu Hu.;Yong-nian Yan.;Zhuo Xiong.;Zhe Wang.;Rong Lu.;Jian-ping Bai.;Jia-ji Yang.
来源: Zhonghua Yi Xue Za Zhi. 2003年83卷7期580-3页
To investigate the effects of collagen I on the adhesion, proliferation, and differentiation of MSCs on PLGA.
6759. [In vitro differentiation of embryonic stem cells mediated by the supernatant of retinal cells and retinoid acid].
作者: Liang Zhang.;Sibo Tang.;Yan Luo.;Bing Huang.;Chun Zhang.;Xigu Chen.
来源: Yan Ke Xue Bao. 2003年19卷2期122-5页
To analyse the differentiation features of embryonic stem cells which were induced by the supernatant of retinal cells and retinoid acid (RA).
6760. [The study on induced differentiation of embryonic stem cells into conjunctival epithelial-like cells].
作者: Danping Huang.;Jian Ge.;Qianying Gao.;Jing Tao.;Jianliang Zheng.
来源: Yan Ke Xue Bao. 2003年19卷2期117-21页
To investigate the probabilities of murine embryonic stem cells (ESC) differentiating into conjunctival epithelial cells and lay a basis for seeking a new source of seed cells provided in conjunctival tissue engineering.
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