To investigate the proliferation and differentiation of neural stem cells after cerebral infarction(CI) in adult rats.

To investigate the anti-damage effect of iminoethyl-lysine on noise-induced cochlea damage in guinea pig.

Mobilization of hematopoietic stem cells from healthy donors by stimulators such as granulocyte colony stimulating factor (G-CSF) in allogeneic peripheral blood stem cell transplantation (allo- PBSCT) is increasingly used clinically; however, the mechanism, the dose, and the schedule are not fully understood. This study was designed to evaluate the effectiveness and safety of single daily dose of 250 microg G-CSF (Lenograstim) for stem cell mobilization and hematopoietic reconstitution after allo-PBSCT.

High dose chemotherapy (HDCT) supported by autologous hematopoietic stem cell transplantation (ASCT) is one of the most effective approaches for the chemo-sensitive lymphoma. The purpose of this study was to investigate the effectiveness of HDCT combined with radiotherapy supported by HSCT in treatment of poor- prognostic moderate-grade and high-grade malignant lymphoma.

It is important to get high quality autologous peripheral blood stem cell (APBSC) for successful peripheral blood stem cell transplantation. Cyclophosphamide (CTX) plus recombined human granulocyte colony-stimulating factor (rhG-CSF) is standard regimen for APBSC mobilization. Etoposide plus rhG-CSF is another regimen for mobilization in recent years. The purpose of this study was to observe and compare the effects of these two regimens in APBSC mobilization for the patients with malignant lymphoma and germ cell tumors.

To study the possibility of curing chronic myeloid leukemia with autogeneic hemopoietic stem cell transplantation in patients with negative Philadelphia (Ph) chromosome induced by imatinib mesylate (STI 571) treatment.

To investigate the alteration of peripheral blood naive T cells in adult patients after hematopoietic stem cell transplantation (HSCT).

To establish a mouse model of acute hematopoietic failure and explore the pathological basis of platelet changes in bone marrow suppression.

To observe the difference in surface morphology and structure between CD34(+) cells from normal human subjects and from patients with leukemia.

Beginning from a mouse EST (GenBank Accession No: BE644537) which was significantly changed in cryptorchidism and represented a novel gene, GeneScan program was performed and a predicted mouse novel gene full-length cDNA sequence containing the BE644537 sequence was attained. Gene-specific primers were designed for PCR in mouse testis cDNA library. The sequencing result of the PCR product showed that we obtain a new gene mTSARG3 (GenBank Accession No: AF419292) whose full cDNA length is 1328 bp containing 8 exons and 7 introns. The predicted open reading frame encodes a protein of 316 amino acid residues. The protein encoded by the new gene is a new member of HSP40 protein family because the sequence contains the highly conserved J domain which is present in all DnaJ-like proteins and is considered to have a critical role in DnaJ-DnaK protein-protein interactions. mTSARG3 protein displays a 46% identity in a 336-amino-acid overlap with DJB4-MOUSE protein. The result of RT-PCR analysis and Northern blot showed that mTSARG3 is specifically expressed in mouse testis. Southern blot showed that there were no loss and rearrangement in cryptorchid. Based upon all these observations, it is considered that the function of the new gene is related to inhibit mouse testis spermatogenesis apoptosis.

It was observed that the spermatogenic cells apoptosis dramatically increased in infertile man. Cloning of novel spermatogenic cell-specific gene related to apoptosis is of momentous physiological and pathological significance to illustrate the apoptosis mechanism and the biology process of spermatogenic cells. A novel mouse gene full-length cDNA sequence-SRG2 was identified (GenBank accession number AF395083), which was significantly changed in cryptorchidism, from a mouse testis cDNA library using a cDNA fragment (GenBank accession number BE644542) as an electronic probe. SRG2 was 1,088 bp in length. The putative protein encoded by this gene was 295 amino acids with a theoretical molecular weight of 33,579 kDa and isoelectric point of 9.64. The sequence shared no significant homology with any known protein in databases except TSARG2, with which its homology was 78%. RT-PCR showed that SRG2 was expressed significantly in testis. Using molecular beacon probe to examine the mRNA expression level of SRG2 gene in cryptorchid testis of various stages, we found that the gene was up-regulated distinctly. Therefore, we conclude that this gene plays an important role in cryptorchid testis.

Two closely linked, highly homologous phenylalanine ammonia-lyase (PAL) genes located in one phage clone, PAL1 and PAL2, were isolated from a wheat genomic library by using a polymerase chain reaction (PCR) fragment of a wheat PAL gene as probe. The two PAL genes were located approximately from 7 kb apart and displayed 93% identity with the same orientation. Southern blot analysis with a PAL1 specific fragment as probe showed the presence of a multiple gene family of PAL in wheat. Northern analyses demonstrated a differential expression of PAL in two Chinese Spring near-isogenic lines upon infection with stem rust fungus Puccinia graminis. In the resistant Chinese Spring Sr11 isogenic line with the resistance gene Sr11 that is known to interact with the avirulence gene P11 from the stem rust fungus, an induced expression of PAL was observed 4 days post inoculation (d.p.i.), and a massive induction was evident 8 d.p.i. By contrast, in the susceptible Chinese Spring sr11 line lacking the resistance gene, induction of PAL was seen 6 d.p.i. and the expression level at 8 d.p.i. was similar to that observed in the resistant line at 6 d.p.i. In wheat suspension culture cells, treatment with either an elicitor isolated from the stem rust fungus or chitin oligomers could activate PAL gene expression within 2 h. The fungal elicitor appeared to be more active in early activation of the PAL gene than were chitin oligomers. These results demonstrated that wheat PAL played an important role in the induced resistance response upon infection with stem rust fungus at transcription level.

To investigate the effects of lithium chloride on the proliferation and apoptosis of human leukemia cell line (K562).

To identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells.

To investigate the mechanisms of in vivo rhG-CSF affecting T lymphocyte.

To explore the feasibility of a new protocol for acute graft versus host disease (aGVHD) prophylaxis in haploidentical bone marrow transplantation.

To investigate the therapeutic effect of conditioning regimen containing fludarabine in nonmyeloablative allogeneic peripheral blood stem cells transplantation (NAST) in the treatment of hematological diseases.

To explore the efficiency and toxicity of non-myeloablative stem cell transplantation (NAST) for hematological disease.

To investigate the level and significance of interleukin-8 (IL-8), soluble intercellular adhesion molecule (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in patients peripheral blood (PB) during mobilization for peripheral blood stem cells harvesting.

To explore the effect of all-trans retinoic acid (RA) on the engraftment of unrelated umbilical cord blood stem/progenitor cell transplantation (UCBT) in murine model.