Observations on structure and distribution of vimentin as determined by indirect immunofluorescence were showed that the arrangement of vimentin intermediate filments (IFs) changed from an extended fibrillar state to a complex cage formation tightly associated with the forming lipid globules during adipose conversion of rat preadipocytes. The effects of differentiation of rat preadipocytes into adipocytes on vimentin mRNA levels as determined by in situ hybridization (ISH) using oligonucleotides probes to vimentin mRNA labeled by digoxigenin (DIG), and on vimentim protein expression as determined by Western blotting were researched. The results suggested that the expressions of vimentin with molecular weight of about 57 kD at the levels of both mRNA and protein was existed throughout the predipocyte differentiation and downregulation of vimentin is required for adipose conversion. The specificity of the association of lipid globules with vimentin IFs during adipose conversion was taken as an example of a possible IF function in relation to a preadipocytes differentiation process, especially discussed as a functional relationship in supporting adiposgenesis.

Human embryonic stem cells can be induced to differentiate into all kinds of cells in vitro to be applied to clinical medicine and scientific research. Because they have to be isolated from human embryos, any attempt to establish human stem cell line is prohibited by religion and ethics in some areas and countries. Parthogenetic embryonic stem cells have similar capacity of totipotency and proliferation, and can be established from parthenogenetic activation of discarded oocytes. This paper reviews the progress in the studies of the establishment of parthenogenetic embryonic stem cell line and its differentiation capacities.

To investigate the effect of gastrodia in extracorporeal oriented inducing the differentiation of mesenchymal stem cells into neuron-like cells.

To investigate patients who had ocular presentations after allogeneic hematopoietic stem cell transplantation.

To investigate the distribution of epidermal stem cells (ESCs) in different degrees of burn wounds in scalded rats.

To isolate and culture human placenta derived adherent cells (hPDAC) and assay their hematopoietic growth factor expression.

To investigate the inhibiting effect of classII major histocompatibility complex transactivator (CIITA) anti-sense cDNA on MHCII molecules expression.

To explore the effect of treatment with immunocyte therapy on benzene-induced haemopoietic dysfunction.

To evaluate the efficacy of treatment with allogeneic peripheral blood stem cell transplantation for benzene-induced severe aplastic anemia.

To study the effect of thyroid hormone on protein kinase C activity and isoprotein expressions in cardiac myocytes and fibroblasts of rats in vitro.

To investigate the mechanism and the suppression effect of human cytomegalovirus (HCMV) on hematopoietic system.

Systematic lupus erythematosis (SLE) is a severe disease which affects the patient for many years and there is no radical cure for the disease. To explore a possible way to treat children with refractory SLE, the authors treated 2 children with grade III and IV lupus nephropathy for 5 years and 7 years respectively, mainly presented with persistent thrombocytopenia, proteinuria, pleural effusion with CD34(+) autologous peripheral progenitor cells transplantation.

To study the clinical features and the incidence of early infectious complications following children hematopoietic stem cells transplantation (HSCT).

Mesenchymal stem cells (MSCs) were adult stem cells which contribute to the regeneration of mesenchymal tissues such as bone cartilage, muscle, ligament, tendon, adipose and stroma. Due to the multipotential ability and self-renewal capacity, the mesenchymal stem cells can be applied in many fields, such as the seed cells in tissues engineering, cell therapy and gene therapy. To enhance the clinical use of MSCs, the investigators studied the isolation and expansion of MSCs from adult bone marrow, fetal bone marrow and human umbilical cord blood, and investigated their biological identities.

The purpose of this article is to discuss the reconstruction of facial muscle defects with tissue engineered myoblast in SD rats.

To observe the heterotopic osteogenesis of autogenous marrow stromal cells loading on porous calcium phosphate ceramic scaffolds with rhBMP2 gene transfection in a Sprague-Dawley rat model.

To establish culture procedures of neural stem cells from embryonic rat brain, determine their stem-cell characteristics and observe the effects of several compounds on their proliferation ability.

To establish a human cervical carcinoma cell line.