To study the effect of various storage methods on cellular compatibility of bio-derived bone.

To clone human Nanog gene and express it in mammalian cells.

To verify the hypothesis that cells with characteristics similar to bone marrow mesenchymal stem cells(MSCs) can be isolated and cultured from human fetal articular cartilage.

To determine transferrin receptor (TfR) expression of human mesenchymal stem cells (MSCs) in vitro and after transplantation in rabbit spinal cord,and to detect implanted MSCs by in vitro autoradiography.

To investigate the new methods of human leukocyte antigen (HLA) mismatched transplantation in patients with hematologic malignancies.

To observe the differentiation and development of skin stem cells on corneal stroma and to discuss the possibility of reconstructing corneal epithelium with skin stem cells.

A total of 181 8-cell embryos and 241 8-cell compacted embryos were collected respectively from C57BL/6 mouse and their cDNA was synthesized by SMART-PCR. Then PCR productions after SSH were cloned into pUCm-T vector according to the size after isolated and purified. Choice the positive clones for sequencing after being confirmed with PCR. All fragment cloned were blastered matching in GenBank for homology analysis. On the base of this work, we select two ESTs (GenBank accession No.: BQ740263 and BQ740251), which were reverse complementary to each other, assemble into a cDNA fragment, which include a whole open read frame (ORF) and submitted to GenBank with an accession number, AY134859. The target fragment were amplified from cDNA of mouse compacted embryos using primers designed according to the ORF and cloned into pUCm-T vector and sequenced. It's confirmed that the gene, Crg1, was the same as AY134859. Crg1 were also blastered matching in GenBank for homology analysis and mapped by database analyses. RT-PCR analysis of Crg1 were done in series mouse embryos, mouse embryo stem cell and mouse embryo fibroblast. We analyzed the expression of Crg1 in 8-cell embryos and 8-cell compacted embryos using semi-quantity RT-PCR. Northern-blot analysis of Crg1 expression in some adult mouse tissues was done too. The results showed that the length of Crg1 is 810 bp. It include only one exon and code a 150 amino acid protein with a theoretical molecular weight of 17670.34 Dalton. The protein is similar to a protein encoded by a known gene, Stella. Crg1 was mapped to chromosome 14 by database analyses. RT-PCR analysis shows that Crg1 expressed in series mouse embryos (2-cell embryos, 4-cell embryos, 8-cell embryos, compacted embryos and blastocyst), and expressed little higher in compacted embryos. Crg1 also expressed in mouse embryo stem cell but not expressed in mouse embryo fibroblast. The gene only expressed weakly in adult mouse ovary, but not expressed in other adult mouse tissues (brain, spleen, heart, skeletal muscle, kidney, testis, epididymis, liver and lung). So, Crg1 may related to compaction in compacted embryos and maintaining cell's pluripotentiality.

To find out why mPEG modification of donor's lymphocytes can attenuate the occurrence of graft versus host disease(GVHD), but not affect the hemopoietic reconstitution of stem/progenitor cells after transplanting the mPEG-modified mononuclear cells from human cord blood into the SCID mice.

To investigate the feasibility of human bone marrow mesenchymal stem cells(hMSCs) as the seed cells for bone and cartilage tissue engineering.

To construct a eukaryotic expression vector containing the extracellular domain of human Jagged1 and Fc fragment of human IgG1 fusion gene and to express the hJagged1(ext)-Fc fusion protein in mammalian cells.

Hematopoietic stem cells (HSC) have the ability of regeneration, differentiation, and reconstructing hematopoietic function, and it is widely used in many fields such as hematopoietic stem cell transplantation, immune therapy, gene therapy and so on. Human cord blood (CB) is abundant of HSC. But a single collection of CB has only a limited amount of HCS and cannot fit the clinical and research use. Thus ex vivo expansion of human CB derived HSC is important. We know that there are some cytokines, which can synergize for enhancing the expansion of CB derived CD34(+) cells in vitro. Currently, some experiments have discovered that IL-6/sIL-6R or its chimera can enhance the ex vivo expansion of CD34(+)gp130+IL-6R- subpopulation. This study was designed to observe the effect of IL-6/sIL-6R on the ex vivo expansion of human CB derived CD34(+) cells, and explore the optimal cytokine combinations.

To study whether human placenta contains hematopoietic stem/progenitor cells (HSPCs), and analyze phenotypes of lymphocyte subpopulations in the placenta.

To study the effect of ex vivo expansion on the migration ability and the CXCR4 expression of umbilical cord blood (CB) hematopoietic stem/progenitor cells (HSPC).

To explore the proliferative capacity of bone marrow hematopoietic stem cells and the function of T helper (Th) lymphocytes of patients with immuno-related pancytopenia (IRP).

To evaluate the efficacy of second allogeneic hematopoietic stem cell transplantation (allo-HSCT) for treatment of leukemia relapsed after first allo-HSCT.

To assess the effect of titanium particles on the proliferation of rMSCs after osteoblastic induction.

To achieve stable and long-term expression of BMP-7 gene in bone marrow stem cells (BMSCs).