当前位置: 首页 >> 检索结果
6481. [Induced differentiation of ectomesenchymal stem cells of human embryo facial process into osteoblast in vitro].
作者: Manjing Deng.;Yan Jin.;Junnan Shi.
来源: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004年18卷4期314-7页
To investigate the possibility of ectomesenchymal stem cell of human embryo facial process in differentiating into osteoblasts.
6482. [Vascularization in transplantation of bio-derived bone compounded with marrow stromal stem cells in repair of goat tibial shaft defect].
作者: Jinli Chen.;Fuguo Huang.;Chao Qi.
来源: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004年18卷4期309-13页
To study the vascularization of the composite of bio-derived bone and marrow stromal stem cells (MSCs) in repairing goat tibial shaft defect.
6483. [Cultivation and differentiation of spinal cord-derived stem cells in vitro in rats].
作者: Dehua Wang.;Jianguang Xu.;Hao Jiang.
来源: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004年18卷4期247-9页
To investigate the method of cultivation and the feature of differentiation of spinal cord-derived stem cells in vitro.
6484. [Factors influencing isolation and cloning of chicken primordial germ cells].
Gonadal primordial germ cells (PGCs) were isolated from 4.5-7.5-day-old chicken embryos. Factors influencing isolation and cloning of chicken PGCs were analyzed in this study. The results attained were as follows: Chicken PGCs proliferated fast in presence of 10% FBS. At primary culture, PGCs were co-cultured with the gonadal stromal cells. For subculture the chicken PGCs were plated with mitotically inactive feeder layer cells. Further research found that the PMEF (primary mice embryonic fibroblast) feeder cells were the most suitable for the growth of PGCs. When the cell culture medium was supplemented with LIF, FGF, SCF and IGF-I, the chicken PGCs were in the best condition. The chicken PGCs were easier to clone when isolated from 5.5-day-old embryonic gonads. In addition, the proper digestion time was 40s when PGCs colonies were passaged.
6485. [Establishment and characterization of a neural stem cell line (RNSC-FMU 1) from rat brain].
作者: Zhi Hong Zheng.;Jian Shi Hu.;Ling Lin.;Xiao Qing You.;Qi Zhang.;Jian Yin Lin.
来源: Shi Yan Sheng Wu Xue Bao. 2004年37卷3期199-204页
A neural stem cell line (RNSC-FMU 1) from rat brain have been established successfully by isolating and culturing neural stem cells from newborn SD rat brain in vitro with free-serum medium and passaging with mechanical division. The cell line cultured can continuously generate in vitro for long-term and it is 21 months (>100 passages) so far. These cells keep the feature of neural stem cell and normal karyotype. These neural stem cells can be induced to differentiate into neurons, astrocytes and oligodendrocytes. The cells have an extensive self-renewal capacity; its doubling time of proliferation is about 20 h. The cells are also cryopreservable. Tumor formation is not observed in nude mice that explanted with the cells. This cell line is a good tool for research of neural stem cell.
6486. [Brain-derived neurotrophic factor induces rat bone marrow stromal cells to differentiate into neuron-like cells in vitro].
作者: Wen Huang.;Cheng Zhang.;Song-lin Chen.;Wei-xi Zhang.;Xiao-li Yao.;Ying Zeng.;Hui Huang.;Shan-wei Feng.;Tai-yun Liu.
来源: Di Yi Jun Yi Da Xue Xue Bao. 2004年24卷8期854-8页
To investigate brain-derived neurotrophic factor (BDNF)-induced differentiation of rat bone marrow stromal cells (MSCs) into neuron-like cells in vitro and observe its neuroprotective effect of BNDF on the differentiated cells, which might provide the better seed cells for treatment of nervous system diseases.
6487. [Transplantation of gene-transfected neural stem cells for transient cerebral ischemia in rats].
作者: Wei Zhu.;Liang-fu Zhou.;Yang Wang.;Jian-hong Zhu.;Ying Mao.
来源: Zhonghua Yi Xue Za Zhi. 2004年84卷12期1029-34页
To detect the expression and the role of vascular endothelial growth factor (VEGF)-transfected neural stem cells (NSCs) in rat brain subjected to ischemia.
6488. [Mobilization of autologous peripheral blood stem cells with etoposide and recombinant human granulocyte colony stimulating factor in malignant tumor patients].
作者: Yuan-kai Shi.;Xiao-hui He.;Xiao-hong Han.;Jian-liang Yang.;Peng Liu.;Chang-gong Zhang.;Bin Ai.
来源: Zhonghua Zhong Liu Za Zhi. 2004年26卷6期360-3页
To observe the combined effect of etoposide (Vp-16) and recombinant human granulocyte colony-stimulating factor (rhG-CSF) on mobilization of autologous peripheral blood stem cells (APBSC) in malignant tumor patients and find out the suitable dose of Vp-16.
6489. [Effect of Chinese drugs on proliferation and differentiation of hemopoietic stem/progenitor cells].6490. [Establishment of a single cell-cloned mouse embryonic stem cell line].
To establish embryonic stem cells culture system and methods.
6491. [Expression changes of protein kinase Calpha during differentiation of mouse embryonic stem cells into neuron-like cells].
作者: Qianying Gao.;Jian Ge.;Zhichong Wang.;Huiyi Chen.;Danping Huang.;Zhaohui Yuan.
来源: Yan Ke Xue Bao. 2004年20卷2期107-12页
To study the expression of protein kinase Calpha (PKCalpha) during differentiation of mouse embryonic stem cells (ESC)into neuron-like cells in an attempt to elucidate their role in signaling.
6492. [In vitro culture and biological characteristics of cranial neural crest stem cell].
作者: Wei-dong Tian.;Hong-bing Jiang.;Lei Liu.;Wei Tang.;Xiao-dong Li.
来源: Hua Xi Kou Qiang Yi Xue Za Zhi. 2004年22卷3期229-31页
According to development biology, cranial neural crest stem cell (CNCSC) can differentiate into precursor cells of tooth, jaw and peripheral nerve system, but in vitro study is less reported. In the present study, CNCSC were dissociated and cultured in vitro, and the biological characteristics of CNCSC was investigated.
6493. [Adipose tissue-derived stromal cells as vector for gene therapy in central nervous system].
作者: Li-ye Yang.;Jia-kun Zheng.;Guo-zhen Hui.;Li-he Guo.
来源: Sichuan Da Xue Xue Bao Yi Xue Ban. 2004年35卷4期463-5页
To investigate exogenous gene expressing ability of adipose tissue-derived stromal cell (ADSCs) and cell distribution after they were transplanted into brains, and to get the genetically modified cells for autografting.
6494. [Recent advance of studies on telomerase function in stem cells].6495. [Differentiation of human fetal liver CD34+ cells into neuronal cells induced by beta-ME and BHA in vitro].
To establish model of differentiation of fetal liver stem cells induced by beta-ME + BHA into neural cells in vitro;
6496. [Effects of hypoxia on the proliferation of embryonic stem cells].
作者: Hui-qing Zhao.;Ling-ling Zhu.;Tong Zhao.;Li-ying Wu.;Ai-shi Ding.;Ming Fan.
来源: Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2004年20卷3期209-13页
To investigate the effects of hypoxia on the proliferation of mouse embryonic stem cells (mouse ES cells) in vitro.
6498. [Expressions of bFGF, ET-1 and SCF in dermal papilla cells and the relation to their biological properties].
作者: Zhong-fa Lu.;Jin-jin Wu.;Rong-qing Liu.;Bai-yu Zhong.;Min Zheng.
来源: Zhejiang Da Xue Xue Bao Yi Xue Ban. 2004年33卷4期296-9页
To investigate the expression of bFGF, ET-1 and SCF in different passages of cultured dermal papilla cells (DPC), and their possible effect on biological behaviour of DPC.
6499. [Serum from partial hepatectomy rat and hepatocyte growth factor stimulate bone marrow cell expressing albumin and alpha fetoprotein].
作者: Jun Ma.;Fang-Ling Duan.;Fu-Gui Yan.;Wen-Xi Li.;Xiao Wang.;Xiang-Yu Chen.;Tian-Hui Gao.;Wu-Ling Zhu.;Zhi-Qing Wang.
来源: Zhonghua Gan Zang Bing Za Zhi. 2004年12卷7期410-3页
To explore the effect of serum from partial hepatectomy (PH) rat and hepatocyte growth factor (HGF) on expression of albumin and AFP of bone marrow cells.
6500. [Effects of growth factors and extracellular matrix on proliferation and differentiation of fetal liver progenitor cell in vitro].
作者: Ai-Lan Qin.;Xia-Qiu Zhou.;Hong Yu.;Qing Xie.;Wei Zhang.;Qing Guo.
来源: Zhonghua Gan Zang Bing Za Zhi. 2004年12卷7期406-9页
Liver development needs a number of growth factors and components of the extracellular matrix. The study is to explore how growth factors and extracellular matrix regulate proliferation and differentiation of fetal liver progenitor cell.
|