To evaluate the feasibility of using fluorescence in situ hybridization(FISH) for the detection of a few common chromosome aneuploidies on interphase nuclei of uncultured amniotic fluid cells.

To clone a novel gene expressed specifically in human embryonic stem cell and to analyze its characteristics.

To establish the mouse model of Gly374Arg mutation in fibroblast growth factor receptor 3(Fgfr3) and to analyze the phenotype of the mutant mice.

To identify herba Visci ovalifolii, Chinese medicinal materials used in Li and Miao nationalities.

To investigate the effects of vascular endothelial growth factor (VEGF) on differentiation and function of dendritic cells derived from CD34+ hematopoietic progenitor cells.

To investigate whether Ginkgo biloba extract can augment endothelial progenitor cell (EPC) number, and promote EPC proliferation, migration and adhesion.

To explore the expression and role of Notch1 gene in the differentiation of the human embryonic neural stem cells to neurons induced by all-trans-retinoic-acid(ATRA).

To analyze the kinetics of hematopoietic cell chimerism in the early period after non-myeloablative stem cell transplantation (NAST) and to investigate the correlation between molecular and hematologic assessment of engraftment or rejection.

To evaluate the efficacy of mitoxantrone combined high dose of cytarabine and recombinant human granulocyte colony-stimulating factor (MAG) regimen for mobilizing autologous peripheral blood stem cells (APBSC) in patients with hematopoietic malignancies.

To establish the best condition of isolation and cultivation of mouse bone marrow mesenchymal stem cells (BMSCs) in vitro, we isolated BMSCs from BALB/C mouse using density gradient centrifugation and adherent selecting. The effects of different centrifuge power, adherent time, serum concentration and cell density on the isolation and cultivation of BMSCs were investigated. The best isolating condition is: 500g x 30min, 24 hours adhering. The best cell density is 12-20 x 10(5)/ml of primary cells and 6.4-25.6 x 10(4)/ml of secondary cells. The best serum concentration is 10%. More than 90% of subcultured cells were adhesive in 8 hours. Thus we have established a cell biological method of isolation and cultivation of BMSCs.

To observe the effect of combined use of jinnaduo (an injection made by extract of Ginkgo leaf, EGb) and Deferoxamine (DFO, a chelating agent) in antagonizing the ototoxicity of cisplatin (CDDP).

To study an effective, safety and convenient method for gene transfer and introduction to inner ear.

Inducing mesenchymal stem cells (MSCs) differentiate to myoblasts with 5-azacytidine(5-Aza-CR), investigating the expression of Myf5 and the role of the signal transduction case of p38 in all the course of differentiation.

To investigate whether puerarin can augment endothelial progenitor cells (EPCs) numbers, promote EPC proliferation, migration and adhesion.

To investigate the effects of neonatal calf serum on the differentiation of human neural stem cells in the hippocampus.

To observe the effect and mechanism of wild-type Smad3 gene on the proliferation and osteoblastic differentiation of rat bone marrow-derived mesenchymal stem cell (MSC).

To investigate the feasibility of differentiation of bone marrow mesenchymal stem cells (MSCs) into skin appandence.

Interleukin-11 (IL-11) is a multifunctional cytokine that directly acts on hematopoietic progenitors, macrophage, T cells, epithelial cells and hepatocytes, as well as promotes hematopoiesis, regulates immunity, protects mucosa and epithelium, and has the effect of anti-inflammatory. Preclinical and clinical studies have demonstrated that IL-11 effectively accelerates the recovery of peripheral blood platelets of acute leukemia patients after chemotherapy. In the hematopoietic stem cell transplantation, IL-11 prevents and treats the graft-versus-host disease leading to the effect of graft-versus-leukemia. Synergizing with G-CSF, IL-11 mobilizes hematopoietic progenitors and stem cells from bone marrow to peripheral blood with higher efficiency. Progress on research and clinical application of IL-11 in treatment of leukemia was reviewed in this paper.

This study was aimed to search for effective cryoprotectants and freezing methods used in cord blood bank (CBB) for cryopreservation of cord blood hematopoietic stem cells. The non-programmed group using 8% final concentration of dimethyl sulfoxide (DMSO) and 5% final concentration hydroxyethyl starch (HES) (molecular weight 120,000) as protectants and group of conventional of programmed controller method using 10% DMSO only as cryoprotectant in cryopreservation of cord blood hematopoietic stem cells were compared. In each of the two groups, 15 cord blood units were used. In non-programmed group, cord blood units put in -80 degrees C refrigerator for 24 hours as a transitional step before deep-freezing in liquid nitrogen, when both of DMSO and HES had been added. The recoveries of the nuclear cells number, the yield of granulocyto-macrophage colony forming units (CFU-GM) and the cells viability in cord blood units before preservation and after thawing were tested for both methods. The results showed that no significant difference was found in above assays between two groups. The clinical application results also showed that hematopoietic engraftment rates after infusion were similar in both groups. It is concluded that the non-programmed method by -80 degrees C refrigerator as a transitional step and using the combined two protectants seems simple in operation and effective in clinical transplantation as well as the conventional programmed method.