To establish rhesus haploidentical hematopoietic stem cell transplantation model by nonmyeloablative conditioning, and examine the effects of mesenchymal stem cells (MSC) in haploidentical transplantation.

To observe the effect of Shuanghuang Shengbai granule on mice leukopenia induced by ip cyclophosphamide (CTX) or radiation.

To study the expession of Nestin after focal ischemia-reperfusion in rats.

To investigate the distribution of epidermal growth factor (EGF) in the CNS of adult rhesus monkey.

To explore the migration and distribution of human neural stem cells (NSCs) after they were transplanted into lateral cerebroventricles of embryonic rats.

The WUS (WUSCHEL) gene encodes a transcription factor that specifies the adjacent cells to be stem cell. The WUS dependent signal systems have been found in different tissues recent years. The feedback loop between the CLV and WUS genes maintains the stem cell self-renewal and apical dominance in shoot apical meristem. In the embryonic meristem, the expression of CLV3 depends on WUS only. During post-embryo development, both WUS and STM are needed for CLV3 expression and the triggering of organogenesis. The expression of AG in floral meristem is activated by co-existence of WUS and LFY, AG acts as a negative regulator of WUS expression to downregulate the WUS level. The signal system established by WUS is also involved in ovule development. The somatic embryogenesis can be promoted efficiently by WUS, especially in the presence of auxin. The results of prevoius works indicated that cell competence to WUS activity is related to microenvironment and the combination of WUS signal with different environmental factors could activate different downstream genes.

To study the genes change of hMSC before and after differentiation into neuron-like cells.

To study the effect of core-binding factor alpha1 (Cbfa1) on the mesenchymal stem cells (MSCs) osteoblastic differentiation.

To explore the in vitro osteogenesis of the chitosan-gelatin scaffold compounded with recombinant human bone morphogenetic protein 2 (rhBMP-2).

To evaluate the effect of nano-hydroxyapatite collagen (nHAC) bone and marrow mesenchymal stem cells (MSCs) on the treatment of rabbit osteonecrosis of the femoral head (ONFH) defect.

To investigate the change of bone marrow cell composition the effect of graft on hemopoietic reconstitution and the incidence of acute graft-versus-host disease (aGVHD) with granulocyte colony-stimulating factor G-CSF administration to donors before marrow harvesting.

To establish clonal human embryonic stem cell lines and investigate their biological characteristics.

To observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang.

To study HBV preS2/S gene expression effects in mammalian cells transferred with recombinant adenoviral vector.

To isolate neural precursor cells from human embryonic cortical tissue of 20 weeks and identify their proliferation capacity as well as differentiating potential.

To explore the feasibility of cord blood transplantation (CBT) for the treatment of adult hematological malignancies and its long-term hematopoiesis reconstitution and transplantation-related complications.

To isolate and identify human retinal stem cells (HRCs) from human embryonic retina.

Severe newborn hypoxic-ischemic encephalopathy (HIE) has a very high rate of disability and no effective treatment is available. The present study aimed to preliminarily evaluate the effects of human neural stem cell transplantation in treatment of severe neonatal HIE.

Neonatal hypoxic-ischemic encephalopathy (HIE) harms the lives and health of newborn infants and children severely. Given the absence of effective therapies for HIE, it is important to derive new strategies. Neural stem cells (NSCs) have great potential as a therapeutic tool for the repair of a number of central nervous system disorders that involve cell loss. This study was designed to transplant the neural stem cells derived from human fetal brain (hNSCs) into cerebral ventricle of neonatal rat following hypoxic-ischemic injury and to investigate their survival, migration and differentiation in rat brain.

To establish a neonatal rat model of hypoxic-ischemic encephalopathy and clarify the changing features of neural stem cells (NSCs) in episodes of hypoxic-ischemic encephalopathy (HIE) so as to provide experimental and theoretical evidences for treating HIE by applying NSCs at the appropraite time.