To constitute a composite skin substitute that can proliferate well with epidermal stem cells and fibroblasts on collagen sponge.

To explore the feasibility of in vivo tracking of bone marrow mesenchymal stem cells (BMSCs) labeled with superparamagnetic iron oxide (SPIO) by magnetic resonance imaging (MRI) in rats after cerebral ischemia, and to analyze the influence of stem cell therapy on the volume of cerebral infarction.

To explore the phenotypic changes of some cell surface antigens in the process that the bone marrow-derived mesenchymal stem cells (MSCs) differentiate into osteoblast lineage under certain conditions.

To investigate the construction of the green fluorescent protein (GFP) labeled recombinant adenovirus containing human stem cell leukemia (hSCL) and its distribution and efficiency in mice with interstitial cells of Cajal (ICC) loss.

To study the effect of high-density lipoprotein (HDL) on the proliferation of endothelial progenitor cells (EPC) isolated from human umbilical cord blood; to further explore its effect on prevention and development of atherogenesis.

Construction of recombinant adenovirus, which contain human microdystrophin, and then transfection into mesenchymal cells( MSCs) of mdx mice were done, and genetically-corrected isogenic MSCs were acquired; the MSCs transplantation into the mdx mice was then done to treat the Duchenne muscular dystrophy( DMD). Microdystrophin cDNA was obtained from recombinant plasmid pBSK-MICRO digested with restrictive endonuclease Not I ; the production was inserted directionally into pShuttle-CMV. The plasmid of pShuttle-CMV-MICRO was digested by Pme I , the fragment containing microdystrophin was reclaimed and transfected into E. coli BJ5183 with plasmid pAdeasy-1. After screening by selected media, the extracted plasmid of positive bacteria was transfected into HEK293 cells with liposome and was identified by observing the CPE of cells and by the PCR method. Finally, MSCs of mdx mice were infected with the culture media containing recombinant adenovirus, and the expression of microdystrophin was detected by RT-PCR and immunocytochemistry. Recombinant adenovirus including microdystrophin was constructed successfully and the titer of recombinant adenovirus was about 5.58 x 10(12) vp/mL. The recombinant adenovirus could infect MSC of mdx mice and microdystrophin could be expressed in the MSC of mdx mice. Recombinant adenovirus including microdystrophin was constructed successfully, and the microdystrophin was expressed in the MSC of mdx mice. This lays the foundation for the further study of microdystrophin as a target gene to correct the dystrophin-defected MSC for stem cell transplantation to cure DMD.

To discuss the feasibility of treating the brain ischemic stroke by the co-transplantation of the neural stem cells(NSCs) and the endothelial progenitor cells(EPCs).

To review the latest development of the research on the self-renwal signaling pathway and culture system in vitro of the embryonic stem cells (ESCs).

To review the development of the liver stem cell transplant for the liver regenerative treatment.

To investigate the research development of the liver stem cell (LSC) and to predict its future application.

To review the development of researches on the stem cells and the tissue engineering technique used in the intestines.

To review the literature about the development of the periodontal tissue engineering.

To investigate whether the implanted myoblasts with the soluble carriers can improve the repairing efficiency for the severely-cryodamaged tibialis anterior muscles.

To investigate the effects of the recombinant human bone morphogenetic protein 2 (rhBMP-2) and/or the osteogenic agents on proliferation and expression of the osteoblast phenotype differentiation of the SD rat mesenchymal stem cells (MSCs).

To investigate the differentiation of the adipose-derived adult stem cell (ADASC) induced by the recombinant adenovirus's containing fibers derived from B-group serotype 35 (rAd5/F35)-mediated human bone morphogenetic protein 7 (hBMP-7) gene and to explore a new cell source for the bone tissue engineering.

To study the properties of the xenogeneic deproteinized cancellous bone used as a scaffold in the bone tissue engineering and its application to the spinal fusion of the lumbar intertransverse process in a goat.

Neural stem cells (NSCs) are proved to be promising cell sources for gene therapy and cell therapy. To pursue optimal conditions for the isolation and culture of neural stem cells residing in human fetal cortex,the cortical tissue was dissociated mechanically and digested with various enzymes. Short-term trypsin digestion combined with pipette dissociation proved to be the suitable method of isolating human NSCs derived from the fetal cortex. Furthermore,DMEM/F12 medium was superior to the neurobasal medium in the aspect of clonal formation. In repeated dissociation experiments,it was found that accutase, instead of trypsin,endowed the NSCs with better growth and efficient neurosphere formation.

To study the cellular compatibility of type I collagen scaffold and human adipose-derived stem cells (ADSC(S)) in order to explore appropriate scaffold materials for adipose tissue engineering.

To observe the role of green fluorescent protein (GFP) in tracing rhesus bone marrow stromal cells (rBMSCs) during tissue-engineered bone formation in vivo.

Previous clinical and experimental results indicate that T-cell immune reconstitution is slow after hematopoietic stem cell transplantation. Immune reconstitution after haploidentical bone marrow transplantation (BMT) is closely related to clinical events. This study was to analyze the repertoire of T-cell receptor beta chain variable region (TCRBV) and the molecular characteristics of T-cell clones during immune reconstitution in leukemia patients after haploidentical BMT.