To evaluate the labeling efficiency of superparamagnetic iron oxide (SPIO) nanoparticles and its toxicity to mouse embryonic stem cells (ESCs) and (embryoid body (ES)-derived cardiomyocytes.

To investigate the effects of Danshen on number and activity of endothelial progenitor cells (EPCs) of patients with Hypercholesterolemia.

To investigate the efficacy of magnetic resonance imaging (MRI) in tracking bone marrow derived mononuclear cells (BM-MNCs) labeled with superparamagnetic iron oxide (SPIO) nanoparticles.

To explore the multidrug resistance (MDR) mechanism of ABC superfamily transporters in the tumor stem cells(TSC) from human brain glioma tissues.

To transfect the recombinant plasmid enhancement type green fluorescent protein C2- neurotrophic factor-3 (pEGFPC2-NT3) into the spinal ganglion cells(SGCs) of guinea pigs' cochlea injured by the excitotoxicity of hydroxyapatite particle (HAT), to inject the recombinant plasmid pEGFPC2-NT3 into the guinea pigs' cochlea, and to observe the expression of pEGFPC2-NT3 and the protective effect of pEGFPC2-NT3 on SGCs of the cochlea in guinea pigs.

To investigate the effect of brain tissue extracts in neonate rats with hypoxic-ischemic brain damage (HIBD) on the differentiation of bone marrow stromal cells (BMSCs) into neural cells.

To apply autologous transplantation of peripheral blood stem cells in the treatment of critical limb ischemia.

To observe the efficacy of Gleevec combined with myeloablative allogeneic stem cells transplantation(Allo-SCT) for the treatment of chronic myeloid leukemia (CML).

To compare the influences of stapedectomy and small fenestra stapedotomy on the hearing of guinea pigs.

The dermal sheath surrounding the outside of the hair follicle maintains and regenerates the dermal papilla, a necessary component for hair regeneration. Dermal sheath cells participate in skin wound healing and have some characteristics of adult stem cells such as immuneprivilege, multiple differentiation and plasticity. It is likely that dermal sheath cells will be the new cynosure in the research on wound healing and tissue engineering. Here we review the studies of hair follicle dermal sheath in present years.

To compare the effects between gene therapy and gradual release carrier for bone morphogenetic protein-2 (BMP-2) in repairing bone defects, bone defects for 15 mm were created.on the bilateral radius in rabbits and treated with four kinds of implantations, ie, composite of transgeneic MSCs and PLA/PCL (Group A), composite of MSCs and gradual release carrier for BMP-2 (Group B), composite of MSCs and PLA/PCL (Group C), and PLA/PCL alone (Group D). After 4, 8, and 12 weeks of the operations, X-ray, histological examination, biomechanics analysis, and bone density measurement were conducted. Results showed that both osteoblasts and mesenchymal cells displayed strongly positive expression of BMP-2 in Group A after 4 weeks of the operation, the speed and quality of bone formation in Group A were much better than those in Group B. After 12 weeks of the operations, bone defects were completely repaired in Group A. BMP-2 gene therapy is really a good method to repair segmental bone defects.

In order to study the effect of ovariectomy on the capacity of rat MSCs' differentiation into adipocytes, we established the animal models of osteoporosis by performing ovariectomy on the 3-months-old and 6-month-old female SD rats. Then MSCs were isolated from these rats by means of the density-gradient centrifugation method. After adipogenic induction (ADI), lipid droplets were detected by oil red O staining, and the mRNA level of lipoprotein lipase(LPL) was measured by RT-PCR assay. The experiments included 8 groups: (1) MSCs from 3-month-old rats (MSCs 3control); (2) MSCs from 3-month-old ovariectomized rats (MSCs 3ovx); (3) MSCs from 6-month-old rats (MSCs 6control); (4) MSCs from 6-month-old ovariectomized rats (MSCs 6ovx); (5) MSCs from 3-month-old rats with ADI (MSCs ADI 3control); (6) MSCs from 3-month-old ovariectomized rats with ADI (MSCs ADI 3ovx); (7) MSCs from 6-month-old rats with ADI (MSCs ADI 6control); 8) MSCs from 6-month-old ovariectomied rats with ADI (MSCs ADI 6ovx). The results showed that the number of lipid droplets and expression level of LPL mRNA in MSCs ADI 3ovx and MSCs ADI 6ovx were higher than those in MSCs ADI 3control and MSCs ADI 6control, respectively; that the number of lipid droplets and expression level of LPL mRNA in MSCs ADI 6control were higher than those in MSCs ADI 3control respectively. The results of this study suggested that the adipogenic potential of MSCs from both aged and ovariectomied osteoporotic rats were augmented.

To observe the growth of rat bone marrow mesenchymal cells (BMMCs) on decellular bovine pericardia in vitro and to investigate the effect of proteins pre-coating on cells retention and proliferation, bovine pericardia were decellularized using trypsin, DNase and Triton X-100 respectively. Then three proteins (fibronetin (FN), gelatin, collagen I) were coated on the surfaces of the bovine pericardia separately. BMMCs were harvested from rat thighbone marrow , then expanded and seeded onto decellular bovine pericardia with the proteins pre-coated . Decelluar bovine pericardia without coating were used as controls. The retention and growth of BMMSCs were observed by Hochest staining and analyzed by MTT method. It was shown that the retention and proliferation of BMMCs on FN group and gelatin group were significantly enhanced comparing with those on collagen I group and control group (P < 0.001). There was no significant difference between FN group and gelatin group (P > 0.05), nor between collagen I group and control group (P > 0.05). We conclude that the retention and proliferation of seeding cells on FN and gelatin could be significantly improved on decellular bovine pericardia (DBP) but not on collagen I.

To determine whether ischemia-induced bone marrow-derived EPCs mobilization is impaired in diabetic mice and the association with vascular endothelial growth factor (VEGF) release post ischemia.

This study assessed cardiac function changes post embryonic stem cells (ESCs) perfusion at different concentrations in the isolated apolipoprotein-E gene deficiency (apo E-/-) and wild type (WT) hearts.

To investigate the effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene (Ad(5)-HGF) on hematopoietic stem cells mobilization in patients with extensive coronary heart disease.

To evaluate the myocardial viability with (201)Tl/(18)F-FDG DISA-SPECT technique in patients with acute myocardial infarction underwent emergent intracoronary autologous bone marrow mononuclear cells (BM-MNC) transplantation.

To observe the effect of fluid shear stress on the eNOS gene expression and NO production in endothelial progenitor cells (EPCs).