Src tyrosine kinase and matrix metalloproteinase play the pivotal roles in lung cancer invasion and metastasis. The aim of this study is to evaluate the effect of Src tyrosine kinase inhibition on secretion of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) by non-small cell lung cancer (NSCLC) cells.

To explore the inhibition effect of RNA interference on the ICP4 expression and DNA replication of herpes simplex virus type 2 (HSV2).

To explore the action mechanism of action of Qingyi Huaji Formula (QYHJ), whether its inhibition on the growth of the tumor is by way of down-regulating Ski expression.

Abundant evidence has suggested that neuroinflammation participates in the pathogenesis of Parkinson's disease (PD). The emerging evidence has supported that microglia may play key roles in the progressive neurodegeneration in PD and might be a promising therapeutic target. Ganoderma lucidum (GL), a traditional Chinese medicinal herb, has been shown potential neuroprotective effect in our clinical trials that lead us to speculate that it might possess potent anti-inflammatory and immunomodulating properties. To test this hypothesis, the present study investigated the potential neuroprotective effect of GL and underlying mechanism through inhibiting microglial activation using co-cultures of dopaminergic neurons and microglia. The cultures of microglia or MES23.5 cells alone or together were treated for 24 h with lipopolysaccharide (LPS, 0.25 μg/mL) as a positive control, GL extracts (50-400 μg/mL) or MES23.5 cell membrane fragments (150 μg/mL) were used in treatment groups. Microglia activation, microglia-derived harmful factors and [(3)H]dopamine ([(3)H]DA) uptake of MES23.5 cells were analyzed. The results showed that microglia were activated by LPS and MPP(+)-treated MES23.5 cell membrane fragments, respectively. Meanwhile, GL extracts significantly prevented the production of microglia-derived proinflammatory and cytotoxic factors, including nitric oxide, tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β), in a dose-dependent manner and down-regulated the TNF-α and IL-1β expressions on mRNA level. In addition, GL extracts antagonized the reduction of [(3)H]DA uptake induced by MPP(+) and microglial activation. In conclusion, these results suggest that GL may be a promising agent for the treatment of PD through anti-inflammation.

To compare the different gene expression profiles among Qingkailing components of BA (baicalin), JA (jasminoidin), CA (cholic acid) and CM (concha margaritiferausta) in regulating hippocampus ischemia related genes of mice.

Our aim was to enhance nisin production by overexpression of nisin immunity gene nisI in nisin-producing strains.

By observing the rats with PDTC after the intervention, myocardial ischemic reperfusion injury (MIRI) during apoptosis, NF-κB and iNOS expression. Analysis of NF-κB and iNOS expression in the rat heart ischemic-reperfusion injury, apoptosis in the process of regulation and its mechanism.

To explore the effect of staphylococcal enterotoxin A(SEA) on CD3(epsilon) chain expression on mononuclear cells in cord blood, which were stimulated by K562 cells.

To investigate the antiproliferation effect of cardamonin (CAR) and its possible mechanisms on human umbilical artery smooth muscle cells (HUASMCs) cultured in the mimicking insulin resistance (IR) medium.

To explore the regulation of Bub1 mRNA expression in endometrial carcinoma cells by estrogen and paclitaxel.

The aim of our work is to investigate the effects of overexpression of two acetyl-CoA synthase genes, ACS1 and ACS2, on the physiological functions of Saccharomyces cerevisiae.

To investigate the influence of di-(2-ethylexyl) phthalate (DEHP) on cell apoptosis and expression of Bcl-2 and bax in cultured human first trimester cytotrophoblasts.

Until now, there are still some questions that chemotherapy could block cancer immunologic escape or not. The aim of this study is to explore the mechanism of paclitaxel in the blocking of immunologic escape in 3LL bearing mice.

The spider venom may inspire new drugs to treat cancer. The aim of this study is to investigate the effects and mechanisms of spider venom on lung adenocarcinoma cell A549.

To study the modulatory effect of Sophora flaveacens and co-administration with Veratrum nigrum on the activity and mRNA expression of cytochrome P450 isoenzymes in rat liver.

to explore the effects of IL-10 on the expression of interferon regulatory factor-1 (IRF-1) after anoxia-reoxygenation in vitro.

to observe the effect of PolyI:C on the expression of TLR3 and inflammation cytokine in the airway smooth muscle cells ASMCs of rat, to investigate relationship between the expression of Toll-like receptor 3 (TLR3) in the airway smooth muscle cells and airway inflammation.

To investigate the effect of dracorhodin perchlorate (DP) on inhibiting high glucose-induced serum and glucocorticoid induced protein kinase 1 (SGK1) and fibronectin (FN) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN) .

By applying 40-400 mmol x L(-1) NaCl, 20-200 mmol x L(-1) NaHCO3, and 5%-30% PEG, this paper studied the effects of saline-alkali and drought stress on the seed germination and Na+/H+ antiporter gene (TvNHX1) expression of Tripolium vulgare. 40-160 mmol x L(-1) NaCl, 20 mmol x L(-1) NaHCO3, and 5%-10% PEG-6000 had less effects on the seed germination; while > or = 240 mmol x L(-1) NaCl decreased the seed germination rate, root length, and shoot length (P < 0.05), > or = 50 mmol x L(-1) NaHCO3 decreased the seed germination rate (P < 0.05), 130 mmol x L(-1) NaHCO3 decreased the seed germination potential, root length, and shoot length (P < 0.05), and > or = 15% PEG delayed the seed germination. TvNHX1 transcripts were basically constitutive at germination stage, but their expression increased distinctly at 160 mmol x L(-1) NaCl, 100 mmol x L(-1) NaHCO3, and 10% PEG. Under salt and drought stress, the expression of TvNHX1 changed in-phase with the phenotype change of germinated seeds, suggesting that TvNHX1 play important roles in the tolerance of T. vulgare against adversity stress.

To investigate the effects of matrine on proliferation, cell cycle, apoptosis rate and beta-catenin-dependent transcriptional activity in cultured hepatoma cell line Hep3B.