To investigate the clinical and laboratory characteristics of various hematopoietic malignant patients with t(3;3)(q21;q26) or inv(3) (q21q26).

Based on our established infectious clone of PRRSV, designated as pCBC2, a series of mutagenesis of 3'-untranslated region (3'-UTR) at primary structure and secondary structure level were constructed. Then the full length mutant clones were transfected into MARC-145 cells, from which the influences of the discrete 3'-UTR mutation on PRRSV replication and transcription were analyzed. The properties of the rescued mutant viruses were then further characterized by Northern Blot and plaque morphology analysis. Our results demonstrated that PRRSV could tolerate more than 41 nucleotides deletion and 23nt insertion in the 3'-UTR, however, minor changes in the conserved stem loop region destroyed virus infectivity. To sum up, the stem-loop structure was essential for virus viability, but 5' end of the 3'-UTR tolerates certain level of nucleotide deletion or insertion. This is the first report to define the essential sequence and secondary structure for PRRSV genome replication and it is useful for future research about the regulation element.

Characters of stem epidermis, leaf epidermis and stoma could be used as important microcosmic morphological characteristic when inheritance trend is studied in Ephedra breeding and identification.

To detect the expression of CD133 in human established larynx tumors cell line, Hep-2 cell line and observe tumorigenicity of CD133(+) tumorigenic cells, CD133(-) tumorigenic cells and unsorted Hep-2 cells in vivo. The marker of cancer stem cells in Hep-2 cell line was explored.

To construct a plasmid expressing short hairpin RNA (shRNA) targeting SMN1 gene in mammalian cells and to investigate the effect of shRNA on the expression of SMN1 gene in human mesenchymal stem cells (hMSCs), thus providing experimental basis for the establishment of SMA cell model.

To study the distribution of bone marrow mesenchymal stem cells (MSCs) in tumor tissue and the possibility of MSCs differentiating into myofibroblast under the induction of local tumor microenvironment.

In this paper are reviewed the current situation and research progress of cartilage tissue engineering, including seeding cell, three-dimensional cytoskeleton, cell culture system and evaluation of artificial cartilage behavior, animal model, and clinical trial.

It is well established that neural stem cells (NSCs) are not randomly distributed throughout the brain, but rather are concentrated around blood vessels. Although NSCs lie in a vascular niche, there is no direct evidence for a functional relationship between the NSCs and blood vessel component cells. It is reported that endothelial cells release soluble factors that stimulate the self-renewal of NSCs, inhibit their differentiation, and enhance their neuron production. Endothelial coculture can activate Notch to promote self-renewal. Furthermore, vascular endothelial growth factor (VEGF) plays a significant role in neural cells; it stimulates the growth and differentiation of astrocytes in the central nervous system (CNS). Therefore, beyond their traditional role as structural components of blood vessels, endothelial cells are not only critical component of the neural stem cell niche, but they also are able to enhance neurogenesis, possibly through the secretion of brain-derived neurotrophic factor.

To investigate the effect of intravenous infusion of rat bone marrow-derived mesenchymal stem cells (MSCs) against lung injuries in neonatal exposed to hyperoxia.

To obtain high expression of human hepatocyte growth factor (HGF) in passaged rabbit bone marrow-derived mesenchymal stem cells (BMSCs) via recombinant adenovirus vector mediated HGF gene transfection, and explore the feasibility of this strategy for local treatment of avascular necrosis of the femoral head (ANFH).

To investigate the feasibility of fabricating tissue engineering skin with human hair follicle bulge cells (HFBCs) to repair full-thickness skin wound.

To explore the feasibility of transplanting the skin from chimeric rats to rabbits.

To investigate the effect of substance P (SP) on the migration and differentiation of epidermal stem cells (ESCs) to hair follicle, and its mechanism.

To explore the effect of melatonin(Mel) on the proliferation, apoptosis and expression of bcl-2 in oxidized low-density lipoprotein(ox-LDL)-induced endothelial progenitor cells (EPC) from human umbilical cord blood in vitro.

To investigate the transfection condition of Type 2 recombinant adeno-associated virus ( rAAV2) in human dendritic cells(DCs) which were induced from the bone marrow CD34+ hematopoietic stem /progenitor cells.

To predict T-cell immune reconstitution by investigating T cell receptor excision circles (TREC) and T-cell receptor beta-chain variable region (TCRBV) clonal repertoire in leukemia patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT).

To study the ectopic osteogenesis and vascularization of the tissue engineered bone promoted by an artificial bone composite that consists of coral hydroxyapatite (CHA), 1,25-(OH)2D3, human marrow stromal osteoblast (hMSO), and human umbilical vein endothelial cell (hUVEC).

To investigate the heterotopic odontogenesis ability of Delta1 gene transfected human dental pulp stem cell (DPSC) and nano-hydroxyapatite/collagen (nHAC) composite scaffold.

To establish a model of the human marrow mesenchymal stem cells (hMSCs) cultured under the hypoxic condition in adults and to investigate the biological features of MSCs under hypoxia.

To investigate the feasibility of a new kind of porous beta tricalcium phosphate (P-TCP) as a scaffold for the bone tissue engineering.