To investigate the effect of allogeneic hemopoietic stem cell transplantation (HSCT) in patients with acute lymphocytic leukemia (ALL) or chronic myelocytic leukemia (CML) resistant to imatinib mesylate (IM).

To evaluate the efficacy of in vivo magnetic resonance imaging (MRI) of mesenchymal stem cells (MSCs) injected intravascularly in treatment of acute renal failure (ARF) , and to investigate the changes of renal function and pathology of ARF after MSC transplantation.

To isolate and culture rat mesenchymal stem cells (MSCs) and induce them to differentiate into photoreceptor cells in vitro.

To investigate in vive osteogenic potential in size-critical bone defect after percutaneous autologous grafting of culture-expanded rabbit autologous BMSCs, osteo-induced BMSCs and combination of both.

To examine the proliferation of the neural progenitor cells in the subventricular zone (SVZ) and around the hematoma after intracerebral hemorrhage (ICH) in adult rats.

To establish a method for culturing and identifying neural stem cells (NSCs) derived from the subventricular zone (SVZ) in adult mice.

To investigate the mineralization capacity of periodontal ligament stem cells (PDLC) by determining the mRNA expressions of alkaline phosphatase (ALP), osteocalcin (OCN) and matrix extracellular phosphoglycoprotein (MEPE) and to explore the potential of MEPE as a differentiation marker for PDLC, and its possible function in PDLC osteogenic differentiation.

To explore whether intra-bone marrow injection strategy could promote the engraftment of human umbilical cord blood derived hematopoietic stem/progenitor cells (HS/PC) in xenotransplanted NOD/SCID mouse model.

To investigate the dynamic changes of intestinal epithelial stem cells during the injured-repaired progress induced by 5-FU.

Patients with irreversible hypothyroidism require lifelong levo-thyroxin ( L-T4) replacement therapy, which makes them feel discomfortable. With the development of the thyroid tissues autotransplantation and embryonic stem cell (ESC), this would be a more physiological approach to the treatment of irreversible hypothyroidism. The animal experiments and human clinical trials on thyroid tissues autotransplantation have shown that the autograft can survive and function. The advanced researches have demonstrated that ESC can differentiate into thyroid follicular cells.

This research tried improving the specificity and efficiency of gene transfection in gene therapy and tried making the liposome a better gene transfer vector to brain by use of the monoclonal antibody (anti-Lex/SSEA-1)-mediated targeting of liposome. The derivatized monoclonal antibody was conjugated to the liposome DOSPER to form the targeting liposome P-MMA-DOSPER. Then, the pEGFP-C2 encapsulated in P-MMA-DOSPER or DOSPER was injected into the lateral ventricle of SD rats respectively, and the brains were taken for frosted slice 1, 3, 7 or 14 days later. The expression of GFP was observed under fluorescent microscope. There was a lot of expression of GFP around the lateral ventricle of rats in each group. But the indirect fluorescence antibody test showed the ratio of GFP+/nestin+ cells to nestin+ cells of every marking time point in the group of P-MMA-DOSPER was higher than the one in the group of DOSPER; the difference was found to be statistically significant (P<0.01). The results proved that the P-MMA-DOSPER can permeat the ependyma and can transfer gene into the nerve stem cells in vivo safely and effectively.

In this study, we collected human umbilical cord blood and separated the autologous serum and clear cells. Then the human mesenchymal stem cells were cultured with the use of autologous serum and fetal serum, respectively. By identifing surface markers with flow cytometry and testing the osteogenic, adipogenic and cardiomyocytes differentiation capacity of the cells, we affirmed that the cultured cells are mesenchymal stem cells. Among 21 cases of umbilical cord blood, 11 cases using fetal bovine serum and 15 cases using autologous successfully cultured mesenchymal stem cells from umbilical cord blood. The UCB-MSCs did not express CD34 and CD45, but they expressed CD29, CD44 and CD105, and they were successfully induced into osteogenic cells, adipogenic cells and cardiomyocytic-like cells. All of these suggest that using autologous serum to culture UCB-MSCs is better than using fetal bovine serum, and the autologous serum cultured UCB-MSCs can also be induced into osteogenic cells, adipogenic cells cardiomycyote-like cells.

A rapid and robust segmentation system is the base of the research on the change process of neural stem cells. In order to improve this system, against the segmentation of the multiple connected and blurred border cells, we present an improved geometric active contour algorithm based on the level set method and the curve evolution theory. This method can solve the topological change by itself and obtain the more real edges of the cells. This method has been adopted in the segmentation of the sequence of neural stem cell. The results prove the validity and veracity.

To compare the transduction efficiencies of adenoviral vector, adeno-associated viral vector, baculoviral vector, and plasmid vector in human bone-marrow-derived mesenchymal stem cells (hBMSCs).

To explore the method for labeling Flk1+ CD31- CD34- human bone marrow mesenchymal stem cells (hBMSCs) with ferumoxide-PLL and evaluate the feasibility of its tracing after transplantation into the brains of Macaca Fascicularis.

To study the expression of CD133, CD34 and CD44 in lung cancer, and distribution of cell subpopulations that generated by combination of different markers.

To explore the effect of nerve growth factor (NGF) on hematopoiesis of granulocyte-macrophage and to elucidate the underlying mechanism.

To investigate the method combining hBMP-9 gene therapy with tissue-engineering techniques to improve osteogenesis in an ectopic bone formation model in rabbits.