To provide an ideal seed cell for tissue engineered urinary bladder and urethra by serially culturing canine smooth muscle cells from urinary bladder in vitro and compare biological characteristics of different passages of cells.

To investigate the effect of bFGF on denervated skeletal muscle in accelerating muscle satellite cell proliferation, supplying neurotrophic factors and reducing muscle atrophy.

The in vitro and in vivo differentiation features of the monoclonal human pancreatic stem cell (mhPSC) line derived from the pancreatic tissues of a male abortive fetus at 4 month-old were studied. The mhPSCs were plated in culture dishes that had been coated with 0.1% gelatin in phosphate-buffered saline without calcium and magnesium. After proliferated for 3 days, the mhPSCs were induced in modified high-glucose Dulbecco's Modified Eagles's Medium for 25 days. The changes of the cell morphology were observed by phasecontrast microscope during inducement course. The results of the mhPSCs in vitro induced to differentiate into functional pancreatic islets were identified using dithizone staining, RT-PCR and stimulation-glucose secreting insulin and C-peptide radioimmunoassay. The mhPSCs suspension was separately injected under the groin hypoderm of male nude mice. On the 30th day, the grafts were taken off. The immunochemistry reactions were performed by the SP method. The in vivo differentiation ability of the mhPSCs in nude mice was assessed. In vitro proliferation culture, the mhPSCs adhesively grew and showed polygon epithelioid morphology. After proliferation a layer, the mhPSCs showed the gravelstone-like. During in vitro directional inducement, the mhPSCs gradually turned from polygon to round, suspended to grow and assembled pancreatic islets-like clusters. On the 15th inducement day, only a few cells of pancreatic islet-like clusters were induced into the beta cells that became crimson with dithizone staining. However, till the 25th inducement day, most cells of pancreatic islet-like clusters had differentiated into the beta cells, as identified by dithizone staining, which expressed transcription factor of insulin. Respectively stimulated with different concentration glucose, the induced pancreatic islets not only secreted insulin and C-peptide, but also the secretion volumes of the insulin and C-peptide were markedly increased after the stimulation with higher concentration glucose (0.01<P<0.05). In the in vivo differentiation experiment, all nude mice which were injected by the mhPSCs displayed a teratoma-like with white color and rich blood vessels. Immunohistochemistry showed that the teratoma-like expressed the proteins of the pdx1, insulin, glucagon, CK, MBP and NF. This indicated that the mhPSCs not only could be in vitro induced into functional islet-like clusters, but also could in vivo differentiate into the pancreatic islets, epithelium and neural cells.

The present study aimed to establish an isolation and culture system for the monoclonal human pancreatic stem cells and monoclonal human pancreatic stem cell line. Some factors which would influence the proliferation of the monoclonal human pancreatic stem cells were assessed. Pancreatic tissues, taken from abortive fetuses by sterile procedures, were dissected into 1 mm3 segments and digested with 0.1% type IV collagenase. The isolated cells were grown in media containing low glucose Dulbecco's Modified Eagles's Medium supplemented with 10% fetal bovine serum (FBS), 3.7 g/L sodium pyruvate, 0.08 g/L penicillin and 0.1 g/L streptomycin. These cells were further digested with 0.25 g/L trypsin and 0.4 g/L EDTA for propagation. The monoclonal human pancreatic stem cells were selected by clone-ring, and further proliferated after addition of 10 ng/mL epidermal growth factor (EGF) in culture media. The cell chromosome set was determined by karyotype analysis. The growth curve was made by the 3-(4, 5)-dimethylthiahiazo (-z-yl)-3, 5-di-phenytetrazoliumromide (MTT) method. The results showed that pancreatic tissues were digested to many single cells and cell clusters with collagenase. Adherently cultured, primary epidermal-like pancreatic stem cells grew clonally. After several times of dissociation and propagation, pancreatic stem cells were gradually purified during generations. Using clone-ring selection, the monoclonal human pancreatic stem cells were obtained. Continuously propagated, a monoclonal human pancreatic stem cell line which was derived from a male abortive fetus of 4 month-old had been passed through 50 generations. Karyotype analysis demonstrated that the chromosome set of the monoclonal human pancreatic stem cell line was normal diploid. Growth curve revealed that monoclonal human pancreatic stem cells grew slowly in initial 1-4 days. Then, they entered the logarithmic growth period in next 5-6 days. Their proliferation was speeded up by supplementation with 15% FBS in culture media, which was even more quickly after addition of the 15 ng/mL EGF or 10 ng/mL insulin growth factor II (IGF-II). The result identified that the monoclonal human pancreatic stem cell line could be obtained by the cell isolation and culture system.

To construct pEGFP-HBx eukaryotic expression plasmid and establish stable and effective transfected rat oval cell (LE/6) strain expressing EGFP-HBx fusion protein to explore the roles of HBx gene and oval cell in carcinogenesis of hepatocellular carcinoma (HCC).

Diabetic cardiomyopathy (DCM), an important cause of heart failure, is characterized by microvascular pathologies and interstitial fibrosis. Bone marrow mesenchymal stem cells (MSCs) are pluripotent, which can differentiate into cardiomyocytes and vascular endothelial cells. They also secrete angiogenic and antiapoptotic factors. However, little information is available about the effect of MSCs transplantation on diabetic heart.

To evaluate the safety and efficacy of intracoronary autologous bone marrow mononuclear cells (BM-MNCs) transplantation in patients with dilated cardiomyopathy (DCM).

The neuropeptid galanin is widely expressed in the central nervous system and has a diverse range of physiological effects including food intaking, arousal/sleep, nociception and reproduction. In this study, expression of galanin and galanin receptors (GalR1 and GalR2) mRNA were identified not only in the neurogenisis regions including subventricular zone (SVZ), rostral migratory stream (RMS) and dentate gyrus (DG) of adult mice but also in the SVZ-derived neural stem cell (NSC) culture. Here, we also showed that the addition of galanin and GalR2-specific agonist Gal2-11 to wild-type or GALKO NSCs under differentiation condition significantly promote the neuritogenesis and increase the length of neurites on the betaIII-tubulin positive cells. This effect could be reduced by treatment of the galanin antagonist M35. These results indicate that galanin and its receptors might regulate neurite extension in differentiating neural stem cells and even participate in the development of the nervous system.

To study the anti-tumor effects and mechanisms of cisplatin(DDP) combined with exosomes.

To explore the possibility of building tissue-engineered adipose tissue and looking for a new approach for the repair of soft tissue defects.

To evaluate the role of human leukocyte antigen G (HLA-G) in the better effect of allogenetic bone marrow transplantation than that of peripheral blood stem cell transplantation.

To investigate the expressions of nestin and vascular endothehal growth factor (VEGF) mRNAs in rat brain tissue after cerebral ischemia-reperfusion injury and their changes in response to Tongxinluo (a traditional Chinese herbal preparation) treatment.

To study the role of extracellular matrix (ECM) in neural differentiation of mouse embryonic stem cells (ESCs).

To study the effects of strychnos alkaloids on the proliferation of adult rat neuroprogenitor cells.

To explore the influence of bone marrow (BM) mesenchymal stem cells (MSCs) on macrophage activation after lipopolysaccharide (LPS) stimulation.

To explore the effect of basic fibroblast growth factor 1 (bFGF1) during embryonic development on hematopoiesis, to study the expression of FGF1, vascular endothelial growth factor receptor (KDR), CD133, CD34 and transcription factors Ihh, SCL, GATA-1, GATA-2 and PU. 1 in the yolk sac, and to learn about the role and relationships of FGF1, hematopoietic cells and transcription factors during embryonic hematopoiesis.

To evaluate the effect of sensitized sera on engraftment of hematopoietic stem cells (HSCs) and its mechanism.

To explore the impact of IL-2- and IL-15-activated donor natural killer (NK) cell infusion on graft-versus-host-disease (GVHD) and graft-versus-leukemia (GVL) effect post allogeneic hematopoietic stem cell transplantation (allo-HSCT).

To investigate the cell composition in granulocyte colony-stimulating factor One (rhG-CSF) primed bone marrow grafts (G-BM) from donors with different characteristics.