Utilizing a gene reporter technique to study the effects of Andrographitis Herba on human CXCR4 and CCR5 promoters.

To study the molecular mechanism of tetramethylpyrazine to induce human promyelocytic HL-60 leukemia cells differentiation.

To investigate the effect of interleukin-17 (IL-17) on the expression of monocyte chemoattractant protein-1(MCP-1) in the primary cultured cardiac myocytes.

To explore the effects of 1, 25(OH)(2);D(3); on parathyroid hormone (PTH) induced transdifferentiation and TGF-β(1); expression in cultured human renal tubular epithelial cells.

Taking the floral buds of 10 years old field-cultivated and 3 years old potted nectarine (Prunus persica var. nectarine cv. Shuguang) as test materials, and by the method of real-time quantitative PCR, this paper studied the expressions of the AQPs genes deltaTIP1 and PIP1; 1 during dormancy and dormancy-release (September 15, 2009-January 15, 2010) and the transcriptional levels of the genes under low temperature stress. Within the period of dormancy and dormancy-release, the transcriptional level of PIP1; 1 presented a persistent increasing trend, and the high level expression of PIP1; 1 in January could be related to the efflux of water through cytoplasma membrane and vacuolar membrane, which protected the bud cells from ice crystal injuries. The contents of soluble sugar, soluble protein, and proline in the bud cells all peaked in January, which prevented the excessive water loss from the cells. After 2 weeks of low temperature treatment, the PIP1; 1 had a high level expression, indicating that it was a cold-induced gene. The transcriptional level of deltaTIP1 fluctuated during dormancy, and increased significantly during dormancy-release, which might be induced by the dormancy-release signals in buds and the resumption of plant activity. After 2 weeks of low temperature treatment, the expression level of deltaTIP1 had no increase, indicating that deltaTIP1 was not a cold-induced gene.

To observe the expression of P-selectin (Ps), intercellular adhesion molecule-1 (ICAM-1) and nuclear factor-kappa B (NF-kappaB) in lung tissues of acute lung injury (ALI) rat model induced by oleic acid (OA) and to explore the protective effects of melatonin (MT) in lung tissues in rats.

To explore the effect of Salvia miltiorrhiza monomer IH764-3 on apoptosis in hydrogen peroxide (H2O2)-stimulated hepatic stellate cells (HSCs).

To investigate the effects of curcumin on the behavior of chronic constrictive injury (CCI) rats and the CX3CR1 expression in spinal cord dorsal horn and dorsal root ganglia (DRG).

To investigate the selective mechanism of dehydroepiandrosterone (DHEA) for osteoblast via ERβ.

To study the effects of intestinal trefoil factor (ITF) on Toll-like receptors (TLR) 2 and 4 expression in intestinal tissue and on intestinal injury in young rats with endotoxemia.

The study was purposed to detect BAFF/APRIL gene expression changes in bone marrow mononuclear cells (BMMNC) and myeloma cell line U266 after interference with glucocorticoid and bortezomib. After separation of BMMNC from 7 patients with multiple myeloma, BAFF/APRIL mRNA expression in BMMNC and U266 cell line was detected by real-time PCR after treated with dexamethasone 100, 200 µg/ml, methylprednisolone 100, 200 µg/ml, bortezomib 0.1 µg/ml alone and dexamethasone or methylprednisolone combined with bortezomib respectively for 48 hours. The results showed that U266 cells and BMMNC of untreated MM patients highly expressed BAFF/APRIL genes. When dexamethasone, methylprednisolone or bortezomib was added to U266 cells or BMMNC alone, BAFF/APRIL gene expression decreased as compared with the blank control (p < 0.01). The inhibiting effect of bortezomib to BAFF/APRIL expression was obviously strong(p < 0.05). When dexamethasone or methylprednisolone combined with bortezomib, the BAFF/APRIL gene expression further decreased compared with dexamethasone or methylprednisolone alone (p < 0.01). As compared with the group of methylprednisolone combined with bortezomib, BAFF/APRIL gene expression decreased in dexamethasone combined with bortezomib with a statistically significant difference (p < 0.05). It is concluded that the expression of BAFF/APRIL gene is down-regulated after bing treated with glucocorticoids and bortezomib, which suggests that besides the glucocorticoid receptor and proteasomes targets, BAFF/APRIL and their receptor sites may be new targets of glucocorticoids and bortezomib.

To explore the effects of excreted/secreted antigens (ESA) of Toxoplasma gondii on CD4+CD25+ Foxp3+ T cells and NK cells of melanoma-bearing mice, as well as the tumor growth.

To observe the effects of paeoniflorin on the expressions of CTGF and PDGF in liver tissue of fibrosis and the serum level of TNF-alpha in mice infected with Schistosoma japonicum, and to explore the protective effect and its mechanisms of paeoniflorin on liver fibrosis.

To explore the effect of anti-psychotic treatment on the expression of Neuregulin-1 (NRG1) mRNA in the peripheral blood lymphocytes of schizophrenia patients.

To investigate the effects of medicated serum prepared with Chinese herbal medicine Zhizhen Recipe (ZZR) on activity of nuclear factor-κB (NF-κB) and expression and function of P-glycoprotein (P-gp) in human colorectal cancer multidrug-resistant cell line HCT-8/VCR.

To study the inhibition of glutathione-s-transferase by total saponins of Panax notoginseng and its kinetics analysis in liver of mice.

To evaluate the effect of tetrandrine (Tet) on nitroglycerin(GTN)-induced activation of the satellite cells released inflammatory cytokines and to explore its mechanism.

To investigate anticancer effect and potential mechanism of tanshinone II(A) (Tan II(A)) on human nasopharyngeal carcinoma cell line CNE cells.

To investigated the effects of isopsoralen on bone marrow stromal stem cells (BMSCs) differentiate and proliferate in vitro.