61. F-18-FDG PET-CT in children and young adults with Ewing sarcoma diagnosed in Norway during 2005-2012: a national population-based study.
作者: Boel Johnsen.;Kjetil Boye.;Karen Rosendahl.;Martin Biermann.;Clement Trovik.;Stein Magnus Aukland.
来源: Clin Physiol Funct Imaging. 2016年36卷6期441-446页
To examine national imaging strategies regarding the use of F-18-FDG PET-CT in patients with Ewing sarcoma and study factors that might influence the use of PET-CT, such as tumour biology (Picci grade of operation specimen), clinical disease stage and age.
62. Cytotoxic response of platinum-coated gold nanorods in human breast cancer cells at very low exposure levels.
作者: Maqusood Ahamed.;Mohd Javed Akhtar.;M A Majeed Khan.;Hisham A Alhadlaq.;Salman A Alrokayan.
来源: Environ Toxicol. 2016年31卷11期1344-1356页
Because of unique optical behavior gold nanorods (GNRs) have attracted attention for the application in biomedical field such as bio-sensing, bio-imaging and hyperthermia. However, toxicological response of GNRs is controversial due to their different surface coating. Therefore, a comprehensive knowledge about toxicological profile of GNRs is necessary before their biomedical applications. First time, we investigated the toxic response of GNRs coated with platinum (GNRs-Pt) in human breast carcinoma (MCF-7) cells. Platinum coating further improves the optical and catalytic properties of GNRs. Assays such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), neutral red uptake (NRU) and lactate dehydroganase (LDH) assays have shown that GNRs-Pt induced cytotoxicity at very low exposure levels (0.1-0.8 μg mL-1 ). Accumulation of cells in SubG1 phase and low mitochondrial membrane potential (JC-1 probe) in treated cells suggest that GNRs-Pt induced cell death via apoptotic pathway. Quantitative real-time PCR data demonstrated that mRNA expression of apoptotic genes (bax, caspase-3 and caspase-9) were up-regulated while anti-apoptotic gene bcl-2 was down-regulated in cells exposed to GNRs-Pt. We further observed the higher activity of caspase-3 and caspase-9 enzymes in GNRs-Pt treated cells supporting mRNA data. Moreover, N-acetyl cysteine (NAC) significantly attenuated the ROS generation and cytotoxicity induced by GNRs-Pt in MCF-7 cells suggesting that ROS might plays a crucial role in GNRs-Pt induced toxicity. This study warns of possible toxicity of GNRs even at very low exposure levels. Further investigations needed to explore potential mechanisms of this low dose toxicity phenomenon. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1344-1356, 2016.
63. Antitumor and antimetastatic effects of pemetrexed-loaded targeted nanoparticles in B16 bearing mice.
作者: Nannan Lu.;Rutian Li.;Qin Liu.;Bing Hu.;Xiaoling Xu.;Chushu Ji.;Xinghua Han.;Pin Wang.;Baorui Liu.
来源: Drug Deliv. 2016年23卷7期2566-2574页
Using nanoparticle delivery for anticancer therapy is a potential new drug modality. We developed a novel gelatinase-stimuli nanoparticle. In this study, we studied the antitumor and antimetastasis effect of pemetrexed-loaded targeted nanoparticles and evaluated the correlation between E-cadherin expression and lung metastasis in subcutaneous xenograft model. Compared with free pemetrexed, pemetrexed-loaded targeted nanoparticles exhibited the best antitumor and antimetastasis efficacy among the four therapeutic groups. The study also indicated that there was an inverse correlation between lung metastasis and E-cadherin expression. These results showed pemetrexed-loaded targeted nanoparticles may be a potent drug for tumor therapy and our preclinical data could provide new direction for clinical therapy of malignant melanoma.
64. Expression, modulation, and clinical correlates of the autophagy protein Beclin-1 in esophageal adenocarcinoma.
Esophageal adenocarcinoma (EAC) is characterized by rapidly increasing incidence and mortality rates and poor survival. Efficacious preventive and treatment options are urgently needed. An increasing number of pharmacologic agents targeting cancer cell death via autophagy mechanisms are being evaluated in hopes of circumventing apoptotic and therapeutic resistance. We report for the first time, loss of Beclin-1, a key mediator of autophagy, was significantly linked to prognostic factors in EAC. Specifically, Beclin-1 expression loss occurred in 49.0% of EAC patients versus 4.8% of controls. There was a significant inverse correlation between loss of Beclin-1 with histologic grade and tumor stage supporting a tumor suppressive role for Beclin-1. Autophagy modulation linked to cell death was examined in EAC cell lines following treatment with a proanthocyanidin-rich cranberry extract, C-PAC, and the commonly used autophagy inducer, rapamycin. C-PAC induced Beclin-1-independent autophagy in EAC cells characterized by reduced phosphorylation at serine 15 and 93, and significant cell death induction. In contrast, rapamycin-induced autophagy resulted in concomitant, increases in total Beclin-1 levels as well as Beclin-1-phosphorylation in a cell line specific manner, leading to long-term cell survival. Furthermore, autophagic LC3-II was induced by C-PAC following siRNA suppression of Beclin-1 in EAC cells. Together these data support a prognostic role of Beclin-1 in EAC with evidence that Beclin-dependent autophagy induction is agent specific. Future studies are necessary to fully interrogate the role autophagy plays in the progression of normal tissue to EAC and how specific agents targeting autophagic mechanisms can be efficaciously applied for cancer prevention or treatment. © 2015 Wiley Periodicals, Inc.
65. Clinicopathological features and outcomes in patients with late recurrence of renal cell carcinoma after radical surgery.
作者: Kazuhiro Kobayashi.;Toshihiro Saito.;Yasuo Kitamura.;Vladimir Bilim.;Tomotaka Toba.;Takashi Kawasaki.;Noboru Hara.;Toshiki Tanikawa.;Yoshihiko Tomita.
来源: Int J Urol. 2016年23卷2期132-7页
To characterize patients experiencing late recurrence after primary radical surgery for renal cell carcinoma and to approach the mechanism of late recurrence.
66. Primary Maligant Melanoma of the Oesophagus.
Primary malignant melanoma of the oesophagus (PMME) is a very rare disease with poor prognosis. The median survival is about 10 months. PMME are highly aggressive biologically and metastasize early via haematogenic and lymphatic pathways. Treatment outcome is poor because malignancy is very advanced at the time of presentation. Here, we present the endoscopic features and management of PMME case.
67. Extreme Thrombocytosis.
作者: S M Itolikar.;S B Salagre.;S Yadav.;D Prabhat.;P V Badhe.
来源: J Assoc Physicians India. 2015年63卷12期70-73页
A 55 years old female presented with hitherto abdominal pain and gangrenous changes of lower limbs. Patient was found to have extreme thrombocytosis. Approach to thrombocytosis is discussed here.
68. Malignant Pericardial Tamponade in a Case of Signet Cell Gastric Carcinoma.
作者: Rakul Nambiar.;Sunil Prasobh Prabhakaran.;Padmakumar Rajasekharan Pillai.;D Dalus.
来源: J Assoc Physicians India. 2015年63卷10期82-3页
We report a case of gastric signet cell carcinoma, presenting as cardiac tamponade, in a young male patient. The diagnosis of gastric signet cell carcinoma was confirmed by immunohistochemistry of the lymph node specimen in our patient.
69. Right Ventricular Myxoma.
作者: S Vadivelmurugan.; Senthamarai.; Sakthimohan.; Janarthanan.; Balanayagam.;Vijay Anand.;K J Venkateswaran.; Ramkumar.; Selvaraj.
来源: J Assoc Physicians India. 2015年63卷10期79-80页
We report a case of 30 year female who presented with complaints of intermittent chest pain and breathlessness for 8 months, Diagnosed to have right ventricular mass protruding into main pulmonary artery during each systole. The mass was completely excised. Histopathological examination showed myxoma.
70. Acute Myeloid Leukemia Presenting as Intracerebral Granulocytic Sarcoma.
The CNS involvement of acute myeloid leukemia (AML) is more commonly manifest as meningeal involvement. Rarely it may present as intravascular tumor aggregates called granulocytic sarcoma which presents as intracranial hemorrhage. We are presenting a case of intracranial, intra-parenchymal granulocytic sarcoma (other names: chloroma, extramedullary myeloblastoma), presenting as acute hemiplegia without cerebral hemorrhage.
71. Clinicopathological Pattern of Penile Cancer in a Tertiary Care Centre in Nepal.
作者: D K Gupta.;B R Luitel.;P R Chalise.;P P Subedi.;S Chapagain.;D K Thakur.;U K Sharma.;P R Gyawali.;G K Shrestha.
来源: JNMA J Nepal Med Assoc. 2015年53卷199期162-5页
Carcinoma of penis is an uncommon entity. The higher incidence in developing country may be because of poor hygiene, less common practice of circumcision and unsafe sexual practice. Timely diagnosis and intervention gives the patient a chance of cure. Data on penile cancer is sparse from Nepal so treatment of penile cancer in our centre is presented here.
72. Giant hepatocellular adenoma with peliosis hepatis in a child: A diagnostic dilemma.
作者: Rajni Yadav.;Saumyaranjan Mallick.;Deepak Mittal.;K S Madhusudan.;Manisha Jana.;Minu Bajpai.;Siddhartha Datta Gupta.;Prasenjit Das.
来源: Trop Gastroenterol. 2015年36卷3期200-2页 73. Advanced gastrointestinal stromal tumors: 10-years experience from a tertiary care centre.
作者: Nida Iqbal.;Atul Sharma.;Nk Shukla.;B K Mohanti.;S V S Deo.;Peush Sahni.;Sujoy Pal.;Sushmita Pathy.;Vinod Raina.;Lalit Kumar.
来源: Trop Gastroenterol. 2015年36卷3期168-73页
Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors of the gastrointestinal tract. We aimed to study the pattern of presentation and treatment outcome of advanced GIST patients seen by us in a 10- year period.
74. An unusual case of histiocytic sarcoma.
作者: Deepti Shukla Misra.;Mukesh Bhardwaj.;V P Bhalla.;Shikha Garg.;Veena Malhotra.
来源: Trop Gastroenterol. 2015年36卷4期261-3页 75. Criteria for designation of clinical substage in canine lymphoma: a survey of veterinary oncologists.
Clinical substage is frequently reported to be prognostic in dogs with lymphoma, yet formal criteria for defining this parameter are lacking. The World Health Organization TNM Classification of Tumors of Domestic Animals simply defines substage as the absence or presence of systemic signs (substages a and b, respectively). We designed a survey to query veterinary oncologists on the criteria they use to determine clinical substage in practice. Gastrointestinal, constitutional and respiratory signs were the most commonly identified clinical factors, with greater than 90% respondents indicating that inappetence, vomiting, diarrhoea, changes in attitude, weakness and dyspnea were integral in assigning clinical substage. Nevertheless, more than three-quarters of respondents also considered metabolic, neurologic and nutritional parameters when making this determination. For most factors, respondents reported mild-to-moderate severity of clinical signs was sufficient for substage b designation.
76. Sentinel node biopsy in patients with intermediate and thick melanomas – A balanced view.
作者: Robyn Saw.;Christopher Allan.;Andrew Barbour.;Sydney Ch'ng.;Brendon J Coventry.;David Gyorki.;Michael Henderson.;Julie Howle.;T Michael Hughes.;Kenneth Lee.;Susan Neuhaus.;Christobel Saunders.;Kerwin Shannon.;Mark Smithers.;David Speakman.;John Spillane.;Jonathan Stretch.
来源: Aust Fam Physician. 2015年44卷12期876页 77. Combination of In Silico Analysis and In Vitro Assay to Investigate Drug Response to Human Epidermal Growth Factor Receptor 2 Mutations in Lung Cancer.
The epidermal growth factor receptor 2 (HER2) has been established as an important target of HER2-positive lung cancer, but somatic mutations in HER2 kinase domain are frequently observed that may cause drug resistance and sensitivity for tyrosine kinase inhibitors (TKIs). In this study, the response profile of 14 small-molecule TKIs upon 11 clinical HER2 mutations was investigated systematically using a synthetic strategy that integrated in silico analysis and in vitro assay to explore the structural basis, energetic property and biological implication underlying the intermolecular interactions of TKIs with wild-type and variant HER2. It is found that most clinical mutations are far away from HER2 active site and thus can only address modest or moderate effect on inhibitor binding. However, few single-point substations such as D769H and D769Y as well as the gatekeeper mutation T798 M were predicted to cause strong resistance for an array of TKIs by reshaping the geometric feature and physiochemical property of the active site. Furthermore, inhibitor response to the most common insertion mutation in HER2 exion 20 (HER2(YVMA) ) was examined in detail; the response can be grouped into three classes: sensitization, resistance and insusceptibility. The Bcr-Abl inhibitor bosutinib and EGFR inhibitor gefitinib were selected as the representatives of, respectively, sensitization and insusceptibility to perform kinase assay against the GST-tagged, recombinant kinase domains of wild-type HER2(WT) and HER2(YVMA) variant. As expected, the biological activity of bosutinib was improved by ∼160-fold due to the insertion, while gefitinib exhibited low inhibitory potency on both HER2(WT) and HER2(YVMA) (IC50 >100 μM). Structural analysis revealed an intensive network of hydrogen bonds and hydrophobic interactions in HER2(YVMA) bosutinib complex, whereas only few nonspecific van der Waals contacts were observed at the complex interface of HER2(YVMA) with gefitinib.
78. [EXPRESSION OF PFKFB, HK2, NAMPT, TSPAN13 AND HSPB8 GENES IN PEDIATRIC GLIOMA].
作者: D O Minchenko.;Y E Novik.;H S Maslak.;O V Tiazhka.;O H Minchenko.
来源: Lik Sprava. 2015年7-8期43-8页
We studied the peculiarity of the expression of several key genes related to dysregulation of cell proliferation and surviving processes in pediatric glioma (glioblastoma multiforme) tissue from five children with age from 5 to 8 years as well a sin corresponding nonmalignant tissue counterparts as control from the same patients. RNA was isolated from glioma tissue and corresponding non-malignant tissue counterparts and PFKFB1, PFKFB2, PFKFB3, PFKFB4, HK2, NAMPT, TSPAN13, and HSPB8 gene expressions were studied by quantitative polymerase chain reaction. It was shown that the expression level of genes PFKFB1, PFKFB2, PFKFB3, PFKFB4, HK2, NAMPT, TSPAN13, and HSPB8 is increased in pediatric gliomas as compared to corresponding non-malignant tissue counterparts, but in different grade. More significant changes were demonstrated for PFKFB3, PFKFB4 HK2, NAMPT, TSPAN13, and HSPB8 genes. Thus, the changes in pediatric glioma tissues of the expression of PFKFB1, PFKFB2, PFKFB3, PFKFB4, HK2, NAMPT, TSPAN13, and HSPB8 genes, which control cell proliferation and apoptosis, possibly contribute to enhance the tumor growth, because these genes control cell proliferation and surviving.
79. DISTAL ILEAL STENOSING SUBSEROSAL LIPOMA: A CASE REPORT.
作者: Emmanuel Innocent.;Kwaghe B Vandhi.;Ajetunmobi Olanrewaju.;Akpa Philip Ojile.;M A Misauno.;Ezra NabasuLemech.; DikkolNendangtokDakup.
来源: Niger J Med. 2015年24卷4期370-3页
Lipoma is the commonest soft tissue tumour and ubiquitous in distribution. The gastrointestinal tract is a rare site for this neoplasm. This 38 years old patient presented to the surgical emergency unit of the Jos University Teaching Hospital with features of intestinal obstruction which was confirmed by plain abdominal X-ray. Patient was resuscitated and had exploratory laparotomy. At surgery, a dilated, oedematous, and pale segment of ileum was seen measuring 56 cm in length and 10 cm short of the ileo-caecal junction, where an obstruction had occurred.The distal segment was collapsed. A limited right hemi-colectomy was done with ileocolic anastomosis. Specimen received atthe Histopathology Laboratory consisted of 45 cm of the ileum, the caecum, appendix, and proximal 25 cm of the colon in continuity. There was stenosis affecting the distal 30 cm of the ileum. The wall of the stenosed part of ileum had intramural fat at the sub-serosal locale. Histology confirmed the presence of sheets of matured adipocytes between the muscularispropria and serosa. Patient condition improved and was discharged seven days after surgery. This case is reported five months after surgery. We recommend that lipoma be at all times considered in the differential diagnosis of intestinal obstruction.
80. RECURRENT PLEOMORPHIC ADENOMA OF THE UPPER LIP: CASE REPORT AND REVIEW OF THE LITERATURE.
Pleomorphic adenoma or mixed tumour is a benign salivary gland tumour, presenting usually in the parotid or submandibular glands. This tumour contains elements of both epithelial and mesenchymal origin. They are the most common tumours (50%), (60-65%) of the major and minor salivary glands. The palate is considered as the most common intraoral site (42.8-68.8%), followed by the upper lip (10.1%) and cheek (5.5%).
|