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261. Development of human induced pluripotent stem cell (iPSC) line from a 60year old female patient with multiple schwannoma.
作者: Shaokun Zhang.;Zhenshan Lv.;Yan Liu.;Qiao Li.;Weiquan Gong.;Lidi Liu.;Hong Wu.
来源: Stem Cell Res. 2017年19卷31-33页
Peripheral blood was collected from a clinically diagnosed 60-year old female patient with multiple schwannoma. Peripheral blood mononuclear cells (PBMCs) were reprogrammed with the Yamanaka KMOS reprogramming factors using the Sendai-virus reprogramming system. The transgene-free iPSC line showed pluripotency verified by immunofluorescent staining for pluripotency markers, and the iPSC line was able to differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This in vitro cellular model will be useful for further pathological studies of multiple schwannoma.
262. New hPSC-based human models to study pediatric Acute Megakaryoblastic Leukemia harboring the fusion oncogene RBM15-MKL1.
作者: Verónica Ayllón.;Marina Vogel-González.;Federico González-Pozas.;Joan Domingo-Reinés.;Rosa Montes.;Lucía Morales-Cacho.;Verónica Ramos-Mejía.
来源: Stem Cell Res. 2017年19卷1-5页
Pediatric Acute Megakaryoblastic Leukemia not associated to Down Syndrome (non-DS AMKL) is a rare disease with a dismal prognosis. Around 15% of patients carry the chromosomal translocation t(1;22) that originates the fusion oncogene RBM15-MKL1, which is linked to an earlier disease onset (median of 6months of age) and arises in utero. Here we report the generation of two hPSC cell lines constitutively expressing the oncogene RBM15-MKL1, resulting in an increased expression of known RBM15-MKL1 gene targets. These cell lines represent new disease models of pediatric AMKL to study the impact of the RBM15-MKL1 oncogene on human embryonic hematopoietic development.
263. Conventional TBNA experience over a 10-year period: Diagnostic yield and associated limitations in a tertiary care government set-up.
It is challenging for pulmonologists to sample mediastinal lymph nodes or some endobronchial lesions because of safety concerns. C-TBNA (conventional transbronchial needle aspiration) is a procedure to sample such sites, but is underutilized. We present a retrospective review of patients subjected to C-TBNA through fibre-optic bronchoscopy over a 10-year period.
264. Molecular fluorescence-guided surgery of peritoneal carcinomatosis of colorectal origin: a single-centre feasibility study.
作者: Niels J Harlaar.;Marjory Koller.;Steven J de Jongh.;Barbara L van Leeuwen.;Patrick H Hemmer.;Schelto Kruijff.;Robert J van Ginkel.;Lukas B Been.;Johannes S de Jong.;Gursah Kats-Ugurlu.;Matthijs D Linssen.;Annelies Jorritsma-Smit.;Marleen van Oosten.;Wouter B Nagengast.;Vasilis Ntziachristos.;Gooitzen M van Dam.
来源: Lancet Gastroenterol Hepatol. 2016年1卷4期283-290页
Optimum cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC) is essential for the curative treatment of peritoneal carcinomatosis of colorectal origin. At present, surgeons depend on visual inspection and palpation for tumour detection. Improved detection of tumour tissue using molecular fluorescence-guided surgery could not only help attain a complete cytoreduction of metastatic lesions, but might also prevent overtreatment by avoiding resection of benign lesions.
265. A non-endoscopic device to sample the oesophageal microbiota: a case-control study.
作者: Daffolyn R Fels Elliott.;Alan W Walker.;Maria O'Donovan.;Julian Parkhill.;Rebecca C Fitzgerald.
来源: Lancet Gastroenterol Hepatol. 2017年2卷1期32-42页
The strongest risk factor for oesophageal adenocarcinoma is reflux disease, and the rising incidence of this coincides with the eradication of Helicobacter pylori, both of which might alter the oesophageal microbiota. We aimed to profile the microbiota at different stages of Barrett's carcinogenesis and investigate the Cytosponge as a minimally invasive tool for sampling the oesophageal microbiota.
266. Risk stratification of Barrett's oesophagus using a non-endoscopic sampling method coupled with a biomarker panel: a cohort study.
作者: Caryn S Ross-Innes.;Hamza Chettouh.;Achilleas Achilleos.;Nuria Galeano-Dalmau.;Irene Debiram-Beecham.;Shona MacRae.;Petros Fessas.;Elaine Walker.;Sibu Varghese.;Theodore Evan.;Pierre S Lao-Sirieix.;Maria O'Donovan.;Shalini Malhotra.;Marco Novelli.;Babett Disep.;Phillip V Kaye.;Laurence B Lovat.;Rehan Haidry.;Michael Griffin.;Krish Ragunath.;Pradeep Bhandari.;Adam Haycock.;Danielle Morris.;Stephen Attwood.;Anjan Dhar.;Colin Rees.;Matt D Rutter.;Richard Ostler.;Benoit Aigret.;Peter D Sasieni.;Rebecca C Fitzgerald.; .
来源: Lancet Gastroenterol Hepatol. 2017年2卷1期23-31页
Barrett's oesophagus predisposes to adenocarcinoma. However, most patients with Barrett's oesophagus will not progress and endoscopic surveillance is invasive, expensive, and fraught by issues of sampling bias and the subjective assessment of dysplasia. We investigated whether a non-endoscopic device, the Cytosponge, could be coupled with clinical and molecular biomarkers to identify a group of patients with low risk of progression suitable for non-endoscopic follow-up.
267. The WNT/β-catenin signaling pathway may be involved in granulosa cell apoptosis from patients with PCOS in North China.
作者: X-Q Wu.;Y-Q Wang.;S-M Xu.;J-F Liu.;X-Y Bi.;Z-Q Wang.;J-P Zhang.
来源: J Gynecol Obstet Hum Reprod. 2017年46卷1期93-99页
Investigate the expressions of WNTs in granulosa cells of PCOS in North China, and explore the possible mechanism.
270. The co-regulators SRC-1 and SMRT are involved in interleukin-6-induced androgen receptor activation.
作者: Qi Wang.;Hui Wang.;Qiang Ju.;Zhen Ding.;Xing Ge.;Qiao-Mei Shi.;Ji-Long Zhou.;Xiao-Long Zhou.;Jin-Peng Zhang.;Mei-Rong Zhang.;Hong-Min Yu.;Li-Chun Xu.
来源: Eur Cytokine Netw. 2016年27卷4期108-113页
The androgen receptor (AR) can be stimulated by interleukin-6 (IL-6) in the absence of androgens to induce prostate cancer progression. The purpose of this study was to investigate whether the co-activator steroid receptor coactivator-1 (SRC-1) and co-repressor silencing mediator for retinoid and thyroid hormone receptors (SMRT) are involved in IL-6-induced AR activation.
271. Local failure and acute radiodermatological toxicity in patients undergoing radiation therapy with and without postmastectomy chest wall bolus: Is bolus ever necessary?
作者: Stephen Abel.;Paul Renz.;Mark Trombetta.;Michael Cowher.;E Day Werts.;Thomas B Julian.;Rodney Wegner.
来源: Pract Radiat Oncol. 2017年7卷3期167-172页
Postmastectomy chest wall radiation therapy has historically used bolus to increase dose at the skin surface. Despite the theoretical benefits of bolus, the clinical implications of locoregional tumor control, cosmesis, and the incidence of radiodermatitis are less well characterized. We hypothesized that treatment in the presence or absence of bolus results in equivalent chest wall recurrence rates, but its presence results in more severe acute dermatologic toxicity.
272. Generation of non-integrated induced pluripotent stem cells from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome.
作者: Dongsheng Guo.;Feima Wu.;Haikun Liu.;Ge Gao.;Shanglong Kou.;Fan Yang.;Nasir Abbas.;Tiancheng Zhou.;Xiujuan Cai.;Hui Zhang.;Dajiang Qin.;Jialiang Li.;Kecheng Xu.;Yin-Xiong Li.
来源: Stem Cell Res. 2017年18卷70-72页
Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G>T mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on all resulted cell lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression and their abilities for differentiating into three germ layers. These iPSC lines provide valuable in vitro resources for pathological study on MEN1 syndrome.
273. Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN.
作者: Dongsheng Guo.;Haikun Liu.;Ge Gao.;Yanli Liu.;Yuanqi Zhuang.;Fan Yang.;Kepin Wang.;Tiancheng Zhou.;Dajiang Qin.;Liangqing Hong.;Jialiang Li.;Kecheng Xu.;Yin-Xiong Li.
来源: Stem Cell Res. 2017年18卷67-69页
A patient specific point mutation (c.1288G>T) of Men1 gene was introduced into wide type iPSC line with CRISPR/Cas9 and single-stranded donor oligonucleotides carrying the mutation. The mutated iPSC line has a heterozygous c.1288G>T mutation on exon-9 of Men1 that was confirmed by sequencing analysis. The karyotype of this line was normal and the pluripotency was demonstrated by its ability to differentiate into three germ layers. These artificially created Men1 mutation in wild type iPSC line will help to dissect out the molecular basis of two patients carried the same mutation from one family who were differentially represented hypoglycemia.
274. Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome.
作者: Dongsheng Guo.;Feima Wu.;Haikun Liu.;Ge Gao.;Shanglong Kou.;Fan Yang.;Nasir Abbas.;Tiancheng Zhou.;Xiujuan Cai.;Hui Zhang.;Dajiang Qin.;Jialiang Li.;Kecheng Xu.;Yin-Xiong Li.
来源: Stem Cell Res. 2017年18卷64-66页
Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G>T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers. This cell line provides an ideal model for studying MEN1.
275. Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene.
作者: Senquan Liu.;Zhaohui Ye.;Yongxing Gao.;Chaoxia He.;Donna W Williams.;Alison Moliterno.;Jerry Spivak.;He Huang.;Linzhao Cheng.
来源: Stem Cell Res. 2017年18卷57-59页
Activating point mutations in the MPL gene encoding the thrombopoietin receptor are found in 3%-10% of essential thrombocythemia (ET) and myelofibrosis patients. Here, we report the derivation of induced pluripotent stem cells (iPSCs) from an ET patient with a heterozygous MPL V501L mutation. Peripheral blood CD34+ progenitor cells were reprogrammed by transient plasmid expression of OCT4, SOX2, KLF4, c-MYC plus BCL2L1 (BCL-xL) genes. The derived line M494 carries a MPL V501L mutation, displays typical iPSC morphology and characteristics, are pluripotent and karyotypically normal. Upon differentiation, the iPSCs are able to differentiate into cells derived from three germ layers.
276. Generation of a human induced pluripotent stem cell (iPSC) line carrying the Parkinson's disease linked LRRK2 variant S1647T.
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with S1647T variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will be useful for further function studies and therapeutic screening.
277. Development of a human induced pluripotent stem cell (iPSC) line from a Parkinson's disease patient carrying the N551K variant in LRRK2 gene.
作者: Dongrui Ma.;Ebonne Yulin Ng.;Li Zeng.;Christina Ying Yan Lim.;Yi Zhao.;Eng King Tan.
来源: Stem Cell Res. 2017年18卷51-53页
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with N551K variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model can complement in vivo PD models for pathophysiological studies and drug screening.
278. Derivation of human induced pluripotent stem cell (iPSC) line with LRRK2 gene R1398H variant in Parkinson's disease.
作者: Dongrui Ma.;Murni Tio.;Shin Hui Ng.; Li Zeng.;Christina Ying Yan Lim.;Yi Zhao.;Eng King Tan.
来源: Stem Cell Res. 2017年18卷48-50页
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 72-year old female Parkinson's disease (PD) patient with R1398H variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model provides a good platform for studying the mechanism of PD, and also for drug testing and gene therapy studies.
279. Reprogramming of a human induced pluripotent stem cell (iPSC) line from a Parkinson's disease patient with a R1628P variant in the LRRK2 gene.
作者: Dongrui Ma.;Wei Zhou.;Ebonne Yulin Ng.;Li Zeng.;Yi Zhao.;Eng King Tan.
来源: Stem Cell Res. 2017年18卷45-47页
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 59-year old male Parkinson's disease (PD) patient with R1628P variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will provide a good resource for further pathophysiological studies of PD.
280. Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells.
作者: John L Mazzilli.;Aleksey Y Domozhirov.;Stacey L Mueller-Ortiz.;Charles A Garcia.;Rick A Wetsel.;Eva M Zsigmond.
来源: Stem Cell Res. 2017年18卷37-40页
The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.
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